Variance in multiplex suspension array assays: microsphere size variation impact.
TLDR
A probable significant cause of variance in suspended microspheres assay results is variation in microsphere diameter, which can potentially be addressed by changes in the manufacturing process.Abstract:
Background
Luminex suspension microarray assays are in widespread use. There are issues of variability of assay readings using this technology.read more
Citations
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Highly multiparametric analysis by mass cytometry.
Olga Ornatsky,Dmitry R. Bandura,Vladimir Baranov,Mark Nitz,Mitchell A. Winnik,Scott D. Tanner +5 more
TL;DR: Mass cytometric immunophenotyping of human leukemia cell lines and leukemia patient samples, differential cell analysis of normal peripheral and umbilical cord blood; intracellular protein identification and metal-encoded bead arrays are described.
Journal ArticleDOI
Antibody-Based Protein Multiplex Platforms: Technical and Operational Challenges
TL;DR: Technical and operational challenges have hindered implementation of multiplexed assays in clinical settings, and formal procedures that guide multiplex assay configuration, analytical validation, and quality control are needed before broad application of severalxed arrays can occur in the in vitro diagnostic market.
Journal ArticleDOI
Survival of encapsulated islets: More than a membrane story
TL;DR: It is highlighted that curing diabetes with a functional bio-artificial pancreas requires optimizing all of these aspects, and that significant advances have already been made in many of them.
Patent
Capture of target dna and rna by probes comprising intercalator molecules
TL;DR: In this article, a technique for specific capture of a single strand target polynucleotide by a complementary probe comprising one or more intercalator molecules is described. But the method is not suitable for the use of intercalators in other applications.
Journal ArticleDOI
Magnetic alginate microspheres: system for the position controlled delivery of nerve growth factor.
Gianni Ciofani,Vittoria Raffa,Arianna Menciassi,Arianna Menciassi,Alfred Cuschieri,Silvestro Micera,Silvestro Micera +6 more
TL;DR: Experiments carried out on PC12 cells with magnetic alginate microspheres loaded with NGF have confirmed the induction of cell differentiation which is strongly dependent on the distance from the microsphere cluster.
References
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Book
The Six Sigma Handbook: A Complete Guide for Green Belts, Black Belts, and Managers at All Levels
TL;DR: In this paper, the authors describe the six-sigma infrastructure, six- Sigma goals and metrics, and how to select and track Six-Sigma Projects, as well as how to maintain control after the project.
Journal ArticleDOI
Six-sigma design (quality control)
TL;DR: The approach to customer satisfaction taken at Motorola is described in this article, which is based on two conclusions derived from various studies: a product is built in the shortest time and at the lowest cost if no mistake is made in the process; and if no defect can be found anywhere in the manufacturing process of building a product for the customer, then the customer probably will not find one either.
Journal ArticleDOI
Analysis of individual data from bead-based assays ("bead arrays").
TL;DR: Individual bead measurements are used here to calculate population parameters integral to the measurement of a bead array to judge ligand binding or other activity.
Journal ArticleDOI
Effect of microsphere binding site density on the apparent affinity of an interaction partner.
TL;DR: In this article, the authors used multiplexed microsphere technology to explore the effect that binding site density has on the apparent affinity of a soluble interaction partner and found that as the immobilized binding sites density of PGC-1α peptide on fluorescent microspheres is increased the measured apparent affinity for PPARγ LBD is increased.