Journal ArticleDOI
Highly multiparametric analysis by mass cytometry.
Olga Ornatsky,Dmitry R. Bandura,Vladimir Baranov,Mark Nitz,Mitchell A. Winnik,Scott D. Tanner +5 more
TLDR
Mass cytometric immunophenotyping of human leukemia cell lines and leukemia patient samples, differential cell analysis of normal peripheral and umbilical cord blood; intracellular protein identification and metal-encoded bead arrays are described.About:
This article is published in Journal of Immunological Methods.The article was published on 2010-09-30. It has received 338 citations till now. The article focuses on the topics: Mass cytometry & Cytometry.read more
Citations
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Journal ArticleDOI
Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum
Sean C. Bendall,Erin F. Simonds,Peng Qiu,El-ad David Amir,Peter O. Krutzik,Rachel Finck,Robert V. Bruggner,Rachel D. Melamed,Angelica Trejo,Olga Ornatsky,Robert S. Balderas,Sylvia K. Plevritis,Karen Sachs,Dana Pe'er,Scott D. Tanner,Garry P. Nolan +15 more
TL;DR: Single-cell “mass cytometry” analyses provide system-wide views of immune signaling in healthy human hematopoiesis, against which drug action and disease can be compared for mechanistic studies and pharmacologic intervention.
Journal ArticleDOI
Mass Cytometry: Single Cells, Many Features.
TL;DR: The current state of mass cytometry is reviewed, providing an overview of the instrumentation, its present capabilities, and methods of data analysis, as well as thoughts on future developments and applications.
Journal ArticleDOI
Standardizing immunophenotyping for the Human Immunology Project
TL;DR: The current state of standardization of flow cytometry assays is outlined, which remains highly variable with regard to sample handling, reagents, instrument setup and data analysis, and the steps required to enable the Human Immunology Project are summarized.
Journal ArticleDOI
Multiplexed ion beam imaging of human breast tumors
Michael Angelo,Sean C. Bendall,Rachel Finck,Matthew Hale,Chuck Hitzman,Alexander D. Borowsky,Richard M. Levenson,John B. Lowe,Scot D. Liu,Shuchun Zhao,Yasodha Natkunam,Garry P. Nolan +11 more
TL;DR: This work has developed a method that uses secondary ion mass spectrometry to image antibodies tagged with isotopically pure elemental metal reporters to provide new insights into disease pathogenesis that will be valuable for basic research, drug discovery and clinical diagnostics.
Journal ArticleDOI
An Immune Atlas of Clear Cell Renal Cell Carcinoma
Stéphane Chevrier,Jacob H. Levine,Vito R T Zanotelli,Karina Silina,Daniel Schulz,Marina Bacac,Carola Ries,Laurie Ailles,Laurie Ailles,Michael A.S. Jewett,Holger Moch,Maries van den Broek,Christian Beisel,Michael B. Stadler,Michael B. Stadler,Craig Gedye,Bernhard Reis,Dana Pe'er,Bernd Bodenmiller +18 more
TL;DR: In this paper, the authors used mass cytometry with extensive antibody panels to perform in-depth immune profiling of samples from 73 clear cell renal cell carcinoma (ccRCC) patients and five healthy controls.
References
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Biomarkers and surrogate endpoints: Preferred definitions and conceptual framework*
Arthur J. Atkinson,Wayne A. Colburn,Victor G. DeGruttola,David L. DeMets,Gregory J. Downing,Daniel F. Hoth,John A. Oates,Carl C. Peck,Robert T. Schooley,Bert A. Spilker,Janet Woodcock,Scott L. Zeger +11 more
TL;DR: Biomarker measurements provide an avenue for researchers to gain a mechanistic understanding of the differences in clinical response that may be influenced by uncontrolled variables (for example, drug metabolism).
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AC133, a Novel Marker for Human Hematopoietic Stem and Progenitor Cells
Amy Yin,Sheri Miraglia,Esmail D. Zanjani,Graça Almeida-Porada,Makio Ogawa,Anne G. Leary,Johanna Olweus,John F. Kearney,David William Buck +8 more
TL;DR: AC133-selected cells engraft successfully in a fetal sheep transplantation model, and human cells harvested from chimeric fetal sheep bone marrow have been shown to successfully engraft secondary recipients, providing evidence for the long-term repopulating potential of AC133(+) cells.
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Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays
Sydney Brenner,Maria Johnson,John Bridgham,George Stefan Golda,David H. Lloyd,Davida Johnson,Shujun Luo,Sarah N. McCurdy,Michael F. Foy,Mark Ewan,Rithy Roth,Dave George,Sam Eletr,Glenn Albrecht,Eric Vermaas,Steven Williams,Keith Moon,Timothy Burcham,Michael C. Pallas,Robert B. Dubridge,James W. Kirchner,Karen L. Fearon,Jen-I Mao,Kevin Corcoran +23 more
TL;DR: A novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 μm diameter microbeads provides an unprecedented depth of analysis permitting application of powerful statistical techniques for discovery of functional relationships among genes.
Journal ArticleDOI
Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry.
Dmitry Bandura,Vladimir I. Baranov,Olga Ornatsky,Alexei Antonov,Robert Kinach,Xudong Lou,Serguei Pavlov,Sergey Vorobiev,John E. Dick,Scott D. Tanner +9 more
TL;DR: A novel instrument for real time analysis of individual biological cells or other microparticles is described and real-time simultaneous detection of multiple isotopes from individual 1.8 microm polystyrene beads labeled with lanthanides is shown.
Journal ArticleDOI
Seventeen-colour flow cytometry: unravelling the immune system
TL;DR: The instrumentation and considers the reagents, analysis and applications for this powerful, new extension of flow-cytometric technology.