Journal ArticleDOI
Virulence Factors of Avian Escherichia coli
Marilda Carlos Vidotto,E. E. Müller,J. C. de Freitas,Amauri Alcindo Alfieri,I G Guimarães,Diógenes Santiago Santos +5 more
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TLDR
The molecular genetic studies of the virulence factors by agarose electrophoresis showed that the plasmids of these strains are of high molecular weight.Abstract:
A total of 45 strains of Escherichia coli isolates from chickens with colisepticemia were examined for virulence factors commonly found in pathogenic groups of E. coli. These strains were studied for the following: pathogenicity in 1-day-old chicks; toxin, hemolysin, and colicin production; cell invasiveness and adherence; hemagglutination for fimbriae detection; serum resistance; aerobactin production in iron-limited conditions; and plasmid content. The characteristics exhibited by virulent strains were invasion for HeLa and chicken fibroblast cells, serum resistance, colicin V, and aerobactin production. None of the isolates were toxigenic or positive in hemagglutination tests. The molecular genetic studies of the virulence factors by agarose electrophoresis showed that the plasmids of these strains are of high molecular weight.read more
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Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts
Francis Dziva,Mark P. Stevens +1 more
TL;DR: High-throughput molecular approaches like signature-tagged transposon mutagenesis have already proved invaluable in revealing portfolios of genes expressed by pathogenic bacteria during infection, and this has enabled identification of APEC O2 factors required for septicaemia in the chicken model.
Journal ArticleDOI
Molecular epidemiology of avian pathogenic Escherichia coli (APEC) isolated from colisepticemia in poultry.
TL;DR: The data indicate only a limited association between the virulence gene pattern and the serogroup of APEC strains and question the sensitivity of O-typing for APEC identification without the application of further diagnostic tools.
Journal ArticleDOI
Characterization of Multiple-Antimicrobial-Resistant Escherichia coli Isolates from Diseased Chickens and Swine in China
Hanchun Yang,Sheng Chen,David G. White,Shaohua Zhao,Patrick F. McDermott,Robert D. Walker,Jianghong Meng +6 more
TL;DR: Findings suggest that multiple-antimicrobial-resistant E. coli isolates, including fluoroquinolone-resistant variants, are commonly present among diseased swine and chickens in China, and they suggest the need for the introduction of surveillance programs in China to monitor antimicrobial resistance in pathogenic bacteria that can be potentially transmitted to humans from food animals.
Journal ArticleDOI
Isolation and characterization of a gene involved in hemagglutination by an avian pathogenic Escherichia coli strain.
D L Provence,rd R Curtiss +1 more
TL;DR: The isolation and characterization of a gene that may be important in the adherence of avian pathogenic Escherichia coli to the avian respiratory tract and the deduced protein was found to contain significant homology to the Haemophilus influenzae and Neisseria gonorrhoeae immunoglobulin A1 proteases are reported.
Journal ArticleDOI
Rapid detection of virulence-associated genes in avian pathogenic Escherichia coli by multiplex polymerase chain reaction.
TL;DR: The multiplex PCR protocol was concluded to be a useful, sensitive, and rapid assay system to detect avian pathogenic E. coli and differentiate them from nonpathogenic strains and those belonging to other pathotypes.
References
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Test for Escherichia coli Enterotoxin Using Infant Mice: Application in a Study of Diarrhea in Children in Honolulu
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Journal ArticleDOI
Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells.
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Journal ArticleDOI
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