Showing papers on "Isopimpinellin published in 2012"

Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp . divaricata (Tenore) Gams. under different light conditions

Abstract: Extracts from the biomass of Ruta graveolens and Ruta graveolens ssp. divaricata cultured in vitro under different light conditions (far-red, red and blue light, UV-A irradiation, in darkness and white light) were tested for the amounts of free phenolic acids and cinnamic acid (twelve compounds) as well as furanocoumarins and umbelliferone (seven compounds) using HPLC methods. Total amounts of the investigated groups of compounds in the cultures of both plants increased from 2.6 to 6.7 times, depending on light quality, and the maximum values reached were 106.50 and 1,276.74 mg 100 g−1 DW (in R. graveolens), and 106.97 and 262.54 mg 100 g−1 DW (in the subspecies), respectively. Both white light and blue light were equally beneficial for the total production of phenolic acids in cultures of both plants, whereas the total production of furanocoumarins was clearly better stimulated by blue light in R. graveolens and by darkness in the subspecies (i.e. the amounts were respectively 1.44 and 1.7 times higher than in the biomass cultivated under white light). The amounts of individual compounds in both plant cultures increased from about 2.2 to 26.3 times depending on light quality. The following bioactive compounds were obtained in quantities which are of interest from a practical perspective: in R. graveolens culture—protocatechuic acid (45 mg 100 g−1 DW), isopimpinellin (about 500 mg 100 g−1 DW) and bergapten (about 270 mg 100 g−1 DW), and in the subspecies culture: p-coumaric acid (70 mg 100 g−1 DW) and isopimpinellin (about 210 mg 100 g−1 DW).

Pressurized Liquid Extraction of Coumarins from Fruits of Heracleum leskowii with Application of Solvents with Different Polarity under Increasing Temperature

Abstract: Coumarins are nowadays an important group of organic compounds from natural sources that are useful in a number of fields. Because they possess different pharmacological properties, finding the proper extraction conditions for their separation from plant matrices is a very important step. In this report Pressurized Liquid Extraction (PLE) under different temperature conditions and with different types of extraction solvents were tested. As a matrix, fruits of Heracleum leskowii have been used. A simple reverse phase high-performance liquid chromatographic method (RP-HPLC) coupled with a photodiode array detector (DAD) has been developed for separation and quantitative analysis of the main coumarins. Umbelliferone, xanthotoxin, angelicin, isopimpinellin, bergapten, imperatorin and isoimperatorin were investigated. Bergapten and imperatorin were dominant in almost all extracts in the range of 9.92 ± 0.02-20.93 ± 0.06 and 12.19 ± 0.98-19.07 ± 0.03 mg/100 g, respectively. Dichloromethane and methanol were chosen as the most proper suitable solvents for extraction of coumarins. By increasing the temperature the amount of extracted coumarins increases in petroleum ether and dichloromethane extracts.

Separation and purification of furanocoumarins from Toddalia asiatica (L.) Lam. using microwave-assisted extraction coupled with high-speed counter-current chromatography.

Abstract: A method of microwave-assisted extraction coupled with high-speed counter-current chromatography was established for separation and purification of isopimpinellin, pimpinellin and phellopterin from Toddalia asiatica (L.) Lam. The conditions of MAE including the extraction solvent, size of sample, solid/liquid ratio, extraction temperature and extraction time were optimized by a mono-factor test. That is, 2.0 g dried powder of T. asiatica (L.) Lam of 0.30-0.15 mm size was extracted with 20 mL (solid/liquid ratio of 1:10, g/mL) methanol under 50 °C for 1 min. The crude extract was separated and purified by high-speed counter-current chromatography with hexane-ethyl acetate-methanol-water (5:5:5.5:4.5, v/v/v/v) solvent system. 0.85 mg/g of isopimpinellin, 2.55 mg/g of pimpinellin and 0.95 mg/g of phellopterin were obtained from original sample in one-step within 240 min, the purity determined by high performance liquid chromatography was 95.0%, 99.1% and 96.4%, respectively. Their chemical structures were further identified by mass spectroscopy and nuclear magnetic resonance spectroscopy. The results demonstrated that microwave-assisted extraction coupled with high-speed counter-current chromatography was a feasible, economical and efficient technique for rapid extraction, separation and purification of effective compounds from natural products.

Biological Activities of Indian Celery, Seseli diffusum (Roxb. ex Sm.) Sant. & Wagh

Abstract: In continuation of our work on Indian celery (Seseli diffusum (Roxb. ex Sm.) Santapau & Wagh; Umbelliferae), the fractionation of the 80% MeOH–H2O extract of the seeds was performed to identify the principles responsible for its folk use as an antispasmodic and diuretic. Several compounds were isolated as active components: seselin (1) and anthriscinol methyl ether (4) showed a selective cytotoxicity to some yeast strains. Compound 1 also showed spasmolytic activity. On the other hand, isopimpinellin (3) and isorutarin (5) exhibited a spasmogenic effect on the smooth muscle preparations. Compound 5 was also found to have antioxidant activity. Among them, compound 4 was isolated for the first time from this plant. Copyright © 2011 John Wiley & Sons, Ltd.

Fitoquímica e quimiossistemática de Euxylophora paraensis (Rutaceae)

Abstract: Phytochemical studies of the leaves and stem have led to the identification of the known coumarins isooxypeucedanin, oxypeucedanin hydrate, xanthotoxin, isopimpinellin, 8-methoxymarmesin and marmesin, flavonoids quercetin-3-O-α-L-rhamnopyranoside, myricetin-3-O-α-L-rhamnopyranoside and hesperidin, alkaloids skimmianine and N-methylflindersine and limonoid limonin. The compounds isolated and the chemical profile of Euxylophora obtained from the literature clearly indicate its phytochemical affinities with other Rutoideae species.

Analytical Methods for Isolation, Separation and Identification of Selected Furanocoumarins in Plant Material

Abstract: The most abundant linear furanocoumarins are psolaren, xanthotoxin, bergapten and isopimpinellin, whereas the angular type is mostly represented by angelicin, sphondin, and pimpinellin. Some structures of furanocoumarins are presented in table 1. As was mentioned for the simple coumarins, numerous minor furanocoumarins have been described in the literature, for example bergamottin (5-geranoxy-psolaren) (Stanley & Vannier, 1967), which has received attention recently as a major grapefruit component interfering with drug metabolism by intestinal CYP3A4 (Bourgaud et al., 2006; Wen et al., 2008).

Isolation and identification of active compound from Radix Angelicae Biseratae extracts against citrus fungus pathogens

Abstract: 【Objective】The objective of the study is to elucidate the activity compounds from Radix Angelicae Biseratae against citrus fungus pathogens.【Method】Antifungal virulence of Radix Angelicae Biseratae ethanol extracts against three kinds of citrus fungus pathogens was measured with growth rate method.Then solvent extraction and silica gel column chromatography was done to separate and purify active ingredients.Fraction B2 was analyzed by GC-MS.【Result】The results showed that ethanol extracts were effective on three kinds of citrus fungus pathogens,in which the best EC50 was 0.289 0 mg·mL-1 against Colletorichum gloeosporioides.Fraction B2 was analyzed to have four coumarins including isobergapten,sphondin,pimpinellin and isopimpinellin.Antifungal virulence of isobergapten,pimpinellin and isopimpinellin against C.gloeosporioides show that EC50 were about 45 mg·L-1,in which pimpinellin was 41.521 6 mg·L-1.Pimpinellin could induce the mycelium branch increase,hypha deformity,partial septum disappear and so on.【Conclusion】Isobergapten,pimpinellin and isopimpinellin were isolated and identified to inhibit citrus fungus pathogens from Radix Angelicae Biseratae ethanol extracts.