Showing papers on "Nucleic acid secondary structure published in 1973"
TL;DR: The estimate of the effect of mismatching on T m values of high polymers is less precise because of the long temperature extrapolation required, but it is estimated that DNA or RNA treated with mutagens which interrupt up to 20% of the nucleotide pairs will show a drop in melting temperature with each unit per cent of modification.
245 citations
TL;DR: Analysis of the Raman spectra of a large number of ribonucleotide monomers and polymers indicates that the frequencies and intensities characteristic of the P-O stretching vibrations provide a basis for the quantitative determination of RNA secondary structure.
111 citations
TL;DR: Electrophoresis in a microscopic fiber separates nucleic acid components in amounts corresponding to 500–1000 pg RNA, which permits RNA base analysis at the cellular level and lends themselves to base analysis of DNA and, with a simple modification, to the simultaneous determination of DNA content and base composition.
Abstract: Electrophoresis in a microscopic fiber (microphoresis) separates nucleic acid components in amounts corresponding to 500–1000 pg RNA (Edstrom 1960 a, 1964 a). This sensitivity permits RNA base analysis at the cellular level. In a scaled- up, somewhat simpler version, cellophane strips are used as the supporting medium (Koenig and BrattgArd, 1963; Ruchel, 1971) for the analysis of 3 000–5 000 pg RNA. Microphoresis in cellophane strips was introduced by Koenig and BrattgArd for the quantitative determination of radioactively labelled RNA, which, with the technique employed, required more RNA than is normally used for fibers. These techniques also lend themselves to base analysis of DNA and, with a simple modification, to the simultaneous determination of DNA content and base composition (Edstrom, 1964 b). Microelectrophoresis can also be used for enzyme determinations, in which substrate and product are separated after incubation with micro-isolated components, as used for intracellular localization of nucleases in starfish oocytes (Sierakowska, Edstrom, and Shugar, 1964).
7 citations