Showing papers on "Penicillium griseofulvum published in 2018"

Metabolic synergies in the biotransformation of organic and metallic toxic compounds by a saprotrophic soil fungus

Abstract: The saprotrophic fungus Penicillium griseofulvum was chosen as model organism to study responses to a mixture of hexachlorocyclohexane (HCH) isomers (α-HCH, β-HCH, γ-HCH, δ-HCH) and potentially toxic metals (vanadium, lead) in solid and liquid media. The P. griseofulvum FBL 500 strain was isolated from polluted soil containing high concentrations of HCH isomers and potentially toxic elements (Pb, V). Experiments were performed in order to analyse the tolerance/resistance of this fungus to xenobiotics and to shed further light on fungal potential in inorganic and organic biotransformations. The aim was to examine the ecological and bioremedial potential of this fungus verifying the presence of mechanisms that allow it to transform HCH isomers and metals under different extreme test conditions. To our knowledge, this work is the first to provide evidence on the biotransformation of HCH mixtures, in combination with toxic metals, by a saprotrophic non-white-rot fungus and on the metabolic synergies involved.

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Abstract: In the present study, an endophytic fungus with a considerable antimicrobial activity was isolated from the medicinal plant Mentha Pulegium L. The morphological and molecular identification revealed that this fungus is a Penicillium griseofulvum. A preliminary screening was done to choose the suitable culture medium for a maximum production of the bioactive compounds using the dual-culture agar diffusion assay. The malt extract agar (MEA) and potato dextrose agar (PDA) media were the suitable, and the MEA was selected for the further study. Extraction was done with three solvents, n-Hexane, dichloromethane and ethyl acetate, and the crude extracts were tested against Gram-positive and negative bacteria. A high activity was found with ethyl acetate and dichloromethane crude extracts against all bacteria with a maximal inhibition zones of 45.5 and 41 mm respectively obtained against Escherichia coli. The minimum inhibitory and bactericidal concentrations (MIC, MBC) of ethyl acetate crude extract were evaluated using the broth micro-dilution method. A MIC of 50 µg / ml on Gram-negative bacteria and of 100 µg / ml on Gram-positive bacteria was found. The MBCS ranged from 50 and 200 µg/ml. The time kill study has revealed a bactericidal activity of the Penicillium griseofulvum crude extract. At 10 h and for all concentrations (MIC, MIC× 2 and MIC× 4), 99.9% killing of the bacterial cells was achieved. These results prove that the extract of Penicillium griseofulvum can be a promising source of important bioactive molecules. Keys words: Antibacterial activity, endophytic fungi, Mentha pulegium L., Penicillium griseofulvum.

Xanthomonas oryzae pv.oryzae antagonizing penicillium griseofulvum Pg-35 strain and fermentation filtrate thereof and application to plant disease control

Abstract: The invention belongs to the field of biotechnological microorganisms, and relates to a Xanthomonas oryzae pv.oryzae antagonizing penicillium griseofulvum Pg-35 strain and the application thereof to plant disease control. The Xanthomonas oryzae pv.oryzae antagonizing penicillium griseofulvum Pg-35 strain is collected in China Center for Type Culture Collection on November 27, 2017, with the collection number of CCTCC M 2017728. A fermentation filtrate of the penicillium griseofulvum Pg-35 strain screened in the invention has a significant inhibitory effect on Xanthomonas oryzae pv.oryzae. Thepenicillium griseofulvum Pg-35 strain can provide a good strain for developing a microbial pesticide and has a relatively good application prospect in biological control of the Xanthomonas oryzae pv.oryzae and the like.

Marine anti-drug-resistant-bacteria active substance, preparation and uses thereof

Abstract: The invention provides Penicillium griseofulvum, which is classified and named Penicillium griseofulvum ZZ380, and is obtained by separating and culturing from a marine animal Pachygrapsus crassipes.According to the present invention, Penicipyrrodiether A is prepared through fermentation with Penicillium griseofulvum, wherein the Penicipyrrodiether A has the activity against methicillin-resistantStaphylococcus aureus, can significantly inhibit the growth of drug-resistant bacteria MRSA, has application prospects in the treatment of infections caused by drug-resistant bacteria MRSA, can be used for preparing drugs against methicillin-resistant Staphylococcus aureus, and has a chemical structural formula defined in the specification.

Composite inoculant, feed or additive and degradation method for aflatoxin

Abstract: The invention relates to the field of microorganisms, in particular to a composite inoculant, a feed or additive and a degradation method for aflatoxin. The composite inoculant includes yeast and penicillium, the yeast is salt resistant candida and/or Saccharomyces rouxii, the penicillium is penicillium griseofulvum and/or penicillium chrysogenum. According to the invention, specific species of yeast and penicillium are combined for use, can effectively degrade aflatoxin, especially can degrade aflatoxin B1 with strong carcinogenicity. According to the invention, the yeast and penicillium arecreatively coordinated for preparation of the composite inoculant, the composite inoculant can be simply treated or added into a feed material to as to reduce the content of aspergillus flavus in feedand benefit the health of raised animals.

Method for producing penicillium griseofulvum, main ingredient of Songgang mycin, through microbial fermentation

Abstract: The invention discloses a method for producing penicillium griseofulvum, a main ingredient of Songgang mycin, through microbial fermentation. The method comprises the following steps: fermenting and culturing penicillium griseofulvum FH1816, to obtain penicillium griseofulvum from a fermented product, wherein the preservation number of the penicillium griseofulvum FH1816 in the China Center for Type Culture Collection is CCTCC NO:M 2018187. The provided penicillium griseofulvum FH1816 is used to produce the penicillium griseofulvum, a material route using no lactose or corn syrup and the novelprocess of feeding in batches, supplementing chlorine and fermenting are adopted, the fermentation potency of the penicillium griseofulvum is greatly improved, the production cost is lowered, and themethod provides reliable material sources for developing the antifungal biopesticide, namely the Songgang mycin.

Strain for producing Songgang mycin main component griseofulvin as well as method

Abstract: The invention discloses a strain for producing a Songgang mycin main component griseofulvin as well as a method. The invention provides penicillium griseofulvum FA868, and the preservation number in the China Center for Typical Culture Collection (CCTCC) is CCTCC NO:M 2018186. The Songgang mycin main component griseofulvin is produced by using the penicillium griseofulvum FA868; the whole processof biologically synthesizing the griseofulvin can be completed without taking lactose and core pulp as main raw materials and through simple seeds and fermentation formula of taking rice as carbon andnitrogen sources and adding a proper amount of inorganic salt; and the characteristics of a high-resistance precursor-chloride is maintained, the total amount of the chloride can reach to above 2.0 percent in the fermentation process, and the dechlorinated griseofulvin content is guaranteed to be below 0.2 percent. A reliable raw material source is provided for developing Songgang mycin serving as anti-fungal biological pesticide.

Griseofulvin-producing strain and application thereof

Abstract: The invention discloses a griseofulvin-producing strain and application thereof. The invention provides Penicillium griseofulvum FH1816, which has a preservation number of CCTCC NO: M 2018187 in ChinaCenter for Type Culture Collection. The Penicillium griseofulvum FH1816 provided by the invention is utilized to produce griseofulvin, a raw material route without using lactose and corn syrup and anew process of fed-batch fermentation with continuous feeding of chlorine are adopted, the fermentation potency of griseofulvin can be greatly increased, and the production cost can be reduced, and areliable raw material source is provided for development of the antifungal biopesticide songgangmeisu.