Showing papers on "Salinispora arenicola published in 2015"
TL;DR: An LC-MS-based metabolomics approach was used to characterise the variation in secondary metabolite production due to changes in the salt content of the growth media as well as across different growth periods (incubation times), and indicated that a 14 day incubation period is optimal for the maximum production of rifamycin B.
Abstract: An LC-MS-based metabolomics approach was used to characterise the variation in secondary metabolite production due to changes in the salt content of the growth media as well as across different growth periods (incubation times). We used metabolomics as a tool to investigate the production of rifamycins (antibiotics) and other secondary metabolites in the obligate marine actinobacterial species Salinispora arenicola, isolated from Great Barrier Reef (GBR) sponges, at two defined salt concentrations and over three different incubation periods. The results indicated that a 14 day incubation period is optimal for the maximum production of rifamycin B, whereas rifamycin S and W achieve their maximum concentration at 29 days. A "chemical profile" link between the days of incubation and the salt concentration of the growth medium was shown to exist and reliably represents a critical point for selection of growth medium and harvest time.
45 citations
TL;DR: An esterase gene, encoding a 325-amino-acid protein (SAestA), was mined form obligate marine actinomycete strain Salinispora arenicola CNP193 genome sequence and showed remarkable stability in presence of organic solvents and log P>2.0 even after incubation for 7 days suggested that SAestA may be a potential candidate for application in industrial processes in aqueous/organic media.
16 citations
TL;DR: The data elucidated Sare0357 biological role and biochemical properties as a Trp and Phe-activating adenylation domain as well as recognized and activated tryptophan (Trp) and phenylalanine (Phe).
Abstract: Salinispora arenicola CNS-205 was a first-isolated obligate marine actinomycete. A gene (sare0357), annotated as ‘‘amino acid adenylation domain’’ located on the genome of Salinispora arenicola CNS-205, was cloned and characterized. The recombinant target protein Sare0357 was expressed in E. coli. Sare0357 specifically recognized and activated tryptophan (Trp) and phenylalanine (Phe). The basic kinetic parameters of Sare0357 for Trp were Km = 0.04 mM, Vmax = 2.1 μM/min, kcat = 14.2 min−1, and for Phe were Km = 0.03 mM, Vmax = 1.6 μM/min, kcat = 10.4 min−1. Our data elucidated Sare0357 biological role and biochemical properties as a Trp and Phe-activating adenylation domain.