Showing papers on "Sodium propionate published in 2003"
TL;DR: The gene expression changes after adaptation to acetate or propionate were not caused solely by uncoupling or osmotic effects but represented specific characteristics of the long-term response of E. coli to either compound.
Abstract: In its natural environment, Escherichia coli is exposed to short-chain fatty acids, such as acetic acid or propionic acid, which can be utilized as carbon sources but which inhibit growth at higher concentrations. DNA microarray experiments revealed expression changes during exponential growth on complex medium due to the presence of sodium acetate or sodium propionate at a neutral external pH. The adaptive responses to acetate and propionate were similar and involved genes in three categories. First, the RNA levels for chemotaxis and flagellum genes increased. Accordingly, the expression of chromosomal fliC'-'lacZ and flhDC'-'lacZ fusions and swimming motility increased after adaptation to acetate or propionate. Second, the expression of many genes that are involved in the uptake and utilization of carbon sources decreased, indicating some kind of catabolite repression by acetate and propionate. Third, the expression of some genes of the general stress response increased, but the increases were more pronounced after short-term exposure for this response than for the adaptive response. Adaptation to propionate but not to acetate involved increased expression of threonine and isoleucine biosynthetic genes. The gene expression changes after adaptation to acetate or propionate were not caused solely by uncoupling or osmotic effects but represented specific characteristics of the long-term response of E. coli to either compound.
118 citations
TL;DR: Propionate infusion decreased meal size similarly for both stages of lactation, but linearly increased intermeal interval for ML but not EL, contrary to the hypothesis that lower milk yield for ML compared with EL decreased glucose demand by the mammary gland and increased the proportion of infused propionate oxidized in the liver forML compared to EL.
90 citations
TL;DR: Observations indicate that the reduction in dietary energy intake from propionate infusion was greater than the energy supplied from infusates, and that propionic acid plays an important role in feed intake regulation by affecting both satiety and hunger.
Abstract: The dose-response effects of intraruminal infusion of propionate on feeding behavior of lactating dairy cows were evaluated with eight ruminally cannulated Holstein cows past peak lactation. Treatments were mixtures of propionic acid and acetic acid containing propionic acid at eight different concentrations in Experiment 1, and mixtures of sodium propionate and sodium acetate containing sodium propionate at 4 different concentrations in Experiment 2. Experimental designs were an 8 x 8 and duplicated 4 x 4 Latin squares, respectively, for Experiments 1 and 2. Treatment solutions were infused into the rumen continuously for 14 h at a rate of 16.7 and 25 mmol/min, respectively, for Experiments 1 and 2. Infusion started 2 h before feeding and ended 12 h after feeding; feeding behavior was monitored for 12 h after feeding using a computerized data acquisition system. Total metabolizable energy (ME) intake was calculated by adding the energy of infusates to dietary energy intake. In both experiments, as the proportion of propionate of the infusates increased, total ME intake and dry matter intake decreased linearly. As infusion of propionate increased, meal size tended (P < 0.09) to decrease linearly and intermeal interval tended (P < 0.07) to increase linearly in Experiment 1; meal size decreased linearly and number of meal bouts tended (P < 0.08) to decrease linearly in Experiment 2. These observations indicate that the reduction in dietary energy intake from propionate infusion was greater than the energy supplied from infusates, and that propionate plays an important role in feed intake regulation by affecting both satiety and hunger.
83 citations
TL;DR: Dietary treatments did not affect dry matter intake (DMI) responses to propionate infusion for either experiment, but propionates infusion decreased DMI linearly in expt.
Abstract: Two experiments were conducted to evaluate how dose-response effects of intraruminal infusion of propionate on feeding behavior and plasma metabolites are altered by diets differing in fermentability. Twelve ruminally cannulated Holstein cows were used in each experiment. Cows were fed diets containing either steam flaked corn or dry cracked corn (30% of dietary dry matter) in expt. 1, and diets differing in forage-to-concentrate ratio (66:34 vs. 36:64) in expt. 2. For both experiments, the experimental design was a crossover for dietary treatment, and a 6 x 6 Latin square for infusion treatment within a diet for each period. Infusion treatments were mixtures of sodium propionate and sodium acetate, containing propionate at 0, 0.2, 0.4, 0.6, 0.8 and 1.0 as a fraction of total volatile fatty acids infused. Treatment solutions were infused into the rumen continuously for 18 h starting 6 h before feeding at a rate of 23.1 mmol/min. Although propionate production from ruminal fermentation was expected to be different, dietary treatments did not affect dry matter intake (DMI) responses to propionate infusion for either experiment. However, propionate infusion decreased DMI linearly in expt. 1, but did not decrease DMI at lower rates of propionate infusion, which were much more effective at increasing plasma glucose concentration in expt. 2. Propionate had a smaller hypophagic effect at low concentrations of plasma glucose and had a greater hypophagic effect at elevated concentrations of plasma glucose, which could be explained by changes in the metabolism of propionate in the liver.
40 citations
TL;DR: In this article, the feasibility of conversion of sodium salts of organic acids (propionic, butyric and octanoic) was demonstrated in water-ethanol media up to 50% v/v.
35 citations
TL;DR: Sodium propionate, as well as sodium butyrate, enhanced the production of recombinant B-domain-deleted, factor VIII (rFVIIIdB) by Chinese hamster ovary cells growing in a spinner-flask with a protein-free medium by more than six-fold.
Abstract: Sodium propionate, as well as sodium butyrate, enhanced the production of recombinant B-domain-deleted, factor VIII (rFVIIIdB) by Chinese hamster ovary cells growing in a spinner-flask with a protein-free medium by more than six-fold. The two acids, however, had different cytotoxicities.
31 citations
Patent•
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TL;DR: A filter for a cigarette which comprises an inorganic basic material and a moisture retaining agent selected from the group consisting of glycerol, sodium propionate and sodium lactate is described in this article.
Abstract: A filter for a cigarette which comprises an inorganic basic material and a moisture retaining agent selected from the group consisting of glycerol, sodium propionate and sodium lactate.
29 citations
TL;DR: Contrary to the hypothesis, ammonium infusion did not reduce hypophagic effects of propionate, possibly because the urea cycle indirectly stimulated oxidative metabolism in the liver by generating oxidizable carbon from amino acid catabolism.
18 citations
TL;DR: The enzyme showed the properties of both a catabolic as well as an anabolic α-ALS, and was optimally active at pH 6.0–8.0, 37 °C and showed concentration-dependent sensitivity to cofactors viz.
Abstract: α-Acetolactate synthase (α-ALS) of Enterobacter cloacae ATCC 27613 was purified to homogeneity by ammonium sulphate precipitation, Sephadex G-200 gel filtration and hydroxyapatite affinity chromatography. The molecular weight of the enzyme was found to be 60 kDa by SDS–polyacrylamide gel electrophoresis and ∼200 kDa by gel filtration through Sephadex G-200, showing that the enzyme is a homotrimer. The Km and Vmax of the enzyme were 20 mM and 200 μmol min−1 mg (protein)−1 respectively. The enzyme was optimally active at pH 6.0–8.0, 37 °C and showed concentration-dependent sensitivity to cofactors viz. FAD, NADP and NADPH and branched chain amino acids: leucine, isoleucine and valine. Substances like sodium formate, sodium acetate and sodium propionate, sugars and the selected intermediates of glycolytic pathway inhibited the enzyme. Glycerol, BSA and pyruvate-TPP stabilized the α-ALS. The enzyme showed the properties of both a catabolic as well as an anabolic α-ALS.
7 citations
Patent•
30 Apr 2003
TL;DR: An additive for the culture medium of photosynthesizing bacteria contains sodium propionate and iron ion salt (10-100 PPM) as mentioned in this paper, which has high reproduction speed, low cost, and friendly to ecological environment.
Abstract: An additive for the culture medium of photosynthesizing bacteria contains sodium propionate and iron ion salt (10-100 PPM). Its advantages are high reproduction speed, low cost, and friendly to ecological environment.
3 citations
Patent•
17 Mar 2003
TL;DR: In this paper, the authors proposed a method for producing photosynthetic bacteria by accelerated culture using a material good for soil and farm products, such as sodium acetate/sodium propionate/magnesium sulfate/ammonium sulfate or beer yeast/dietary fiber/mineral as a food additive approved by Food Sanitation Law.
Abstract: PROBLEM TO BE SOLVED: To provide a method for producing photosynthetic bacteria by accelerated culture using a material good for soil and farm products, such as sodium acetate/sodium propionate/magnesium sulfate/ammonium sulfate or beer yeast/dietary fiber/mineral as a food additive approved by Food Sanitation Law, i.e. a material for purple non-sulfur bacteria as a member of photosynthetic bacteria good for vitalizing soil. SOLUTION: The method for producing the photosynthetic bacteria involves using the material such as sodium acetate/sodium propionate/magnesium sulfate/ammonium sulfate or beer yeast/dietary fiber/mineral as the food additive approved by Food Sanitation Law, i.e. the material for purple non-sulfur bacteria as a member of photosynthetic bacteria good for modifying soil declined in activity as a result of depression attributable to the use of chemical fertilizers or agrochemicals. The material is commercially available, easy to procure, and enables the culture of the photosynthetic bacteria to be accelerated, and even if farm products, or the like, which have absorbed nutrients from the relevant soil are eaten, there is no concern for human health, therefore the material seems to be suitable for use in the organic farming field in the future. COPYRIGHT: (C)2005,JPO&NCIPI
Patent•
29 Aug 2003
TL;DR: In this article, an improving composition of the "superfort" type, for bread and bakery products, consisting of 0.2 -30% ascorbic acid and 1...70% wheat gluten and/or 1...50% guar gum, was proposed.
Abstract: The invention relates to an improving composition of the "superfort" type, for bread and bakery products, consisting of 0.2...30% ascorbic acid and 1...70% wheat gluten and/or 1...50% guar gum and/or 1...50% carboxyl methylcellulose, to which there can be added up to 20% dextrose, up to 15% starch, up to 20% malt extract, up to 80% monodiglycerides, up to 70% esters of monodiglycerides with organic fatty acids such as acetic acid, lactic acid,citric acid, tartaric acid, mono-acetyltartaric acid, diacetyltartric acid, up to 60% stearoyl 2-calcium or sodium lactylate, up to 80% lecithin, up to 20% soybean flour or soybean protein concentrate, up to 30% calcium acetate, up to 20% calcium or sodium propionate, up to 15% sorbic acid or potassium sorbate or sorbitol, up to 15% citric acid, up to 20% acetic acid, up to 20% lactic acid, up to 20% calcium carbonate, up to 20% calcium phosphate, up to 20% calcium sulphate, up to 60% alpha-amylase or malt flour, up to 60% xylanase, up to 70% glucoxydase, up to 65% lipase, up to 70% lipoxygenase, up to 60% protease, up to 50% papain, up to 60% transglutaminase, up to 30% glutathione, up to 30% cysteine and up to 50% white wheat flour, the percentage being expressed in weight.