Adeline Goulet

TL;DR: It is demonstrated that both Acr families from S. thermophilus phages inhibit Cas9-mediated genome editing of human cells and is compared to those of other Acrs, including AcrIIA5, and characterize their effectiveness against a range of CRISPR-Cas systems.

TL;DR: The structures indicate that allosteric inhibitors of human kinesin-5, which are being developed as anti-cancer therapeutics, bind to a motor conformation that occurs in the course of normal function, however, due to evolutionarily defined sequence variations in L5, this conformation is not adopted by invertebrate kinesIn-5s, explaining their resistance to drug inhibition.

TL;DR: The contribution of kinesin-5–specific variations to motor function is identified indicating that kinesins indeed are precisely tuned according to cellular function, and multiple adaptations within kinesIn motor domains that tune their mechanochemistries according to distinct functional requirements are highlighted.

TL;DR: The model can potentially reconcile a number of previous, apparently conflicting, observations and proposals for the reversal mechanism of yeast kinesins-5 and shows that the direction of Cut7-driven microtubule sliding can be reversed by crowding it with non-Cut7 proteins.

TL;DR: It is found that St1Cas9 strain variants enable targeting to five distinct A-rich PAMs and provide a structural basis for their specificities.