Adison Wong

TL;DR: A modified version of the synthetic genetic system, including a gene encoding an anti-biofilm enzyme, is generated and the engineered probiotic shows in vivo prophylactic and therapeutic activity against P. aeruginosa during gut infection in two animal models (Caenorhabditis elegans and mice).

TL;DR: P pH-sensing riboswitches are designed to create a gene expression program, digitalize the system to respond to a narrow pH range and apply it to evolve host cells with improved tolerance to a variety of organic acids.

TL;DR: This work describes a representative case study for the debugging of genetic context-dependent effects through principles elucidated herein, thereby providing a rational design framework to integrate multiple genetic circuits in a single prokaryotic cell.

TL;DR: The de novo production of odd chain‐length fatty aldehydes and fatty alcohols in Saccharomyces cerevisiae is demonstrated by expressing a novel biosynthetic pathway involving cytosolic thioesterase, rice α‐dioxygenase and endogenous aldehyde reductases, which has the potential to enable sustainable and efficient synthesis of fatty acid‐derived chemicals from processed biomass.

TL;DR: A 'design-build-test' framework to expedite microbiome engineering efforts is discussed by reviewing current literature on three key aspects: design principles to engineer the human microbiome, methods to engineer microbiome with desired functions, and analytical techniques to examine complex microbiome samples.