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TL;DR: In this article, a 96-well assay that integrates the enrichment of EVs by antibody-coated magnetic beads and the electrochemical detection, in less than one hour of total assay time, of EV-bound proteins after enzymatic amplification was presented.

TL;DR: The results indicate that single MV analysis is likely necessary to identify rare tumoral MV populations and the single vesicle analytical technique used here can be applied to both MV and exosome fractions without the need for their separation from each other.

TL;DR: The exPTN composition of EVs and RNPs reveals that they are closely related by vesicle type, independent of their cellular origin, and single EV analysis reveals that small EVs are less heterogeneous in their protein content than larger ones.

TL;DR: A new separation approach, known as dual‐mode chromatography (DMC), capable of enriching plasma EVs, and depleting LPPs is reported, and the two‐in‐one operation is fast (15 min per sample) and equipment‐free.

TL;DR: This work reports an imaging approach that recovers the full electric field of fluorescent light with single-molecule sensitivity and expands the principle of digital holography to fast fluorescent detection by eliminating the need for phase cycling and enable three-dimensional tracking of individual nanoparticles with an in-plane resolution of 15 nm and a z-range of 8 mm.