Alberto Estevez

TL;DR: The mouse I105N/human I104N mutation, which has been shown to prevent macrophage pyroptosis, attenuated both cell killing by p30 in a 293T transient overexpression system and membrane permeabilization in vitro, suggesting that the mutants are actually hypomorphs, but must be above certain concentration to exhibit activity.

TL;DR: A novel protein-engineering strategy is devised that overcomes the technical complexities of producing full-length human Nav channels and demonstrates how this important new class of gating modifier engages VSD4 to inhibit Nav channel activity through a “voltage-sensor trapping” mechanism.

TL;DR: An in-vivo enrichment technique is described that identifies broadly neutralizing influenza A antibodies with high frequency and elicit robust in vivo synergism when combined with oseltamivir, thus highlighting treatment strategies that could benefit influenza-infected patients.

TL;DR: It is shown that the consensus E1 binding sequence is a close match to a conserved tripeptide motif present in all Wnt inhibitors that bind LRP5/6, providing a detailed structural explanation for the effect of the BMD mutations.

TL;DR: Biochemical analyses of receptor mutants in the context of a high-resolution ECD structure show that this previously unrecognized inhibitory activity of the ECD involves an interaction with the third extracellular loop of the receptor and suggest that glucagon-mediated structural changes in the E CD accompany receptor activation.