Claudio Ciferri

TL;DR: It is reported here that Ndc80/Hec1 functions in regulating kinetochore microtubule plus-end dynamics and attachment stability, and a key role for the Hec1 N terminus is revealed in controlling dynamic behavior of kinetic microtubules.

TL;DR: The mouse I105N/human I104N mutation, which has been shown to prevent macrophage pyroptosis, attenuated both cell killing by p30 in a 293T transient overexpression system and membrane permeabilization in vitro, suggesting that the mutants are actually hypomorphs, but must be above certain concentration to exhibit activity.

TL;DR: An engineered "bonsai" Ndc80 complex containing a shortened rod domain but retaining the globular domains required for kinetochore localization and microtubule binding is crystallized, revealing a microtubules-binding interface containing a pair of tightly interacting calponin-homology (CH) domains with a previously unknown arrangement.

TL;DR: The structure represents an intermediate state of activation of Aurora B in which the Aurora B C-terminal segment stabilizes an open conformation of the catalytic cleft, and a critical ion pair in the kinase active site is impaired.

TL;DR: The recent advances in the field are described and critically assesses their relevance for drug discovery as well as discussing at what stages of the drug discovery pipeline cryo-EM can be useful today and what to expect in the near future.