Allen C. Eaves

TL;DR: It is shown that the lesser effect of imatinib mesylate on the 3-week output of cells produced in vitro from lin−CD34+CD38− CML (stem) cells compared with cultures initiated with the CD38+ subset of lin− CD34+ cells is markedly enhanced when conditions of reduced growth factor stimulation are used.

TL;DR: The development of a nontoxic and reproducible method for detaching the adherent layer and making it into a single-cell suspension suitable for characterization by colony assays was found to provide an appropriate methodology and emphasize the importance of assessing the progenitor content of the adherence layer of long-term human marrow cultures.

TL;DR: A dominant negative regulatory role of endogenously produced TGF-beta is indicated in unperturbed LTC, with activation of primitive hematopoietic cells being achieved by mechanisms that stimulate stromal cells to produce G- CSF, GM-CSF, and IL-6.

TL;DR: The previously reported enhancing effect of human leukocyte conditioned medium on burst formation seen in cultures of human nonadherent cells was found to be due largely to an effect on the formation of the largest, late appearing type of burst.

TL;DR: Support is provided for the view that early erythropoietic cell differentiation involves a series of changes that take place long before competence to synthesize hemoglobin becomes manifest.