A
Ambarish Biswas
Researcher at University of Otago
Publications - 32
Citations - 1596
Ambarish Biswas is an academic researcher from University of Otago. The author has contributed to research in topics: CRISPR & Trans-activating crRNA. The author has an hindex of 12, co-authored 30 publications receiving 1162 citations. Previous affiliations of Ambarish Biswas include National University of Singapore & AgResearch.
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Journal ArticleDOI
Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival.
Chris Greening,Ambarish Biswas,Carlo R Carere,Colin J. Jackson,Matthew C. Taylor,Matthew B. Stott,Gregory M. Cook,Gregory M. Cook,Sergio E. Morales +8 more
TL;DR: It is predicted that this hydrogenase diversity supports H2-based respiration, fermentation and carbon fixation processes in both oxic and anoxic environments, in addition to various H1N1-sensing, electron-bifurcation and energy-conversion mechanisms.
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CRISPRTarget: bioinformatic prediction and analysis of crRNA targets.
TL;DR: A tool is provided that predicts the most likely targets of CRISPR RNAs and is used to discover targets in newly sequenced genomic or metagenomic data, and features and targets of well-characterized Streptococcus thermophilus and Sulfolobus solfataricus type II and IIICRISPR/Cas systems are discovered.
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CRISPRDetect: A flexible algorithm to define CRISPR arrays
TL;DR: A new approach to automatically detect, predict and interactively refine CRISPR arrays, which enables more accurate detection of arrays and spacers and its gff output is suitable for inclusion in genome annotation pipelines and visualisation.
Journal ArticleDOI
Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR-Cas system.
Raymond H.J. Staals,Simon A. Jackson,Ambarish Biswas,Stan J. J. Brouns,Chris M. Brown,Peter C. Fineran +5 more
TL;DR: It is demonstrated that interference-driven spacer acquisition (‘targeted acquisition') is a major contributor to adaptation in type I-F CRISPR–Cas systems and acquisition of self-targeting spacers is occurring at a constant rate in wild-type cells and can be triggered by foreign DNA with similarity to the bacterial chromosome.
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H2 metabolism is widespread and diverse among human colonic microbes
TL;DR: It is suggested that electron-bifurcation rather than respiration is the dominant mechanism of H2 reoxidation in the human colon, generating reduced ferredoxin to sustain carbon-fixation (e.g. acetogenesis) and respiration (via the Rnf complex).