Showing papers by "Amy E. Pasquinelli published in 2005"
TL;DR: It is revealed that mRNAs containing partial miRNA complementary sites can be targeted for degradation in vivo, raising the possibility that regulation at the level of mRNA stability may be more common than previously appreciated for the miRNA pathway.
1,356 citations
TL;DR: Considering the intense genetic efforts applied to understanding development, it is surprising that a relatively large class of regulatory genes has newly surfaced and implicated specific microRNAs in complex genetic pathways regulating embryos, hematopoiesis, neuronal differentiation and Hox-mediated development.
338 citations
TL;DR: It is demonstrated that some of these genes are also required for germline and somatic transgene silencing, and the physical interactions among these potential RNAi factors suggest links to other RNA-dependent gene regulatory pathways.
Abstract: RNA interference (RNAi) of target genes is triggered by double-stranded RNAs (dsRNAs) processed by conserved nucleases and accessory factors. To identify the genetic components required for RNAi, we performed a genome-wide screen using an engineered RNAi sensor strain of Caenorhabditis elegans. The RNAi screen identified 90 genes. These included Piwi/PAZ proteins, DEAH helicases, RNA binding/processing factors, chromatin-associated factors, DNA recombination proteins, nuclear import/export factors, and 11 known components of the RNAi machinery. We demonstrate that some of these genes are also required for germline and somatic transgene silencing. Moreover, the physical interactions among these potential RNAi factors suggest links to other RNA-dependent gene regulatory pathways.
294 citations
TL;DR: It is reported that lin‐41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways and expressed in the chick limb consistent with the hypothesis that they regulate chicken Lin‐41 activity in vivo.
Abstract: We have cloned the chicken and mouse orthologues of the Caenorhabditis elegans heterochronic gene lin-41. During limb development, lin-41 is expressed in three phases over developmental time and most notably is associated with the developing autopod. Using chicken and mouse mutants and bead implantations, we report that lin-41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways. In C. elegans, it is proposed that lin-41 activity is temporally regulated by miRNAs (let-7 and lin-4) that bind to complementary sites in the lin-41 3′-untranslated region (UTR). Taking a bioinformatics approach, we also report the presence of potential miRNA binding sites in the 3′-UTR of chicken lin-41, including sites for the chicken orthologues of both C. elegans let-7 and lin-4. Finally, we show that these miRNAs and others are expressed in the chick limb consistent with the hypothesis that they regulate chicken Lin-41 activity in vivo. Developmental Dynamics 234:948–960, 2005. © 2005 Wiley-Liss, Inc.
65 citations