Ana del Pozo-Rodríguez

TL;DR: By modifying critical physicochemical parameters of the NLC formulation, this work was able to overcome the P-gp drug efflux and alter the transcytosis mechanism of the nanoparticles, which support the use of NLCs approaches for oral delivery of poorly water-soluble P- gp substrates.

TL;DR: This manuscript includes a revision of the strategies employed to enhance in vitro transcribed mRNA functionality and efficacy, including the optimization of its stability and translational efficiency, as well as the regulation of its immunostimulatory properties.

TL;DR: In spite of the high DNA condensation capacity of protamine and its content in NLS, this does not always lead to an improvement in cell transfection since it may impair some of the limiting steps of the transfections processes.

TL;DR: The therapeutic plasmid which encodes the protein Retinoschisin was employed achieving a positive transfection in ARPE-19 cells, showing a promising application of this new non-viral system for the treatment of X-linked juvenile retinoschisis by gene therapy.

TL;DR: Scientific results are summarized that evidence the potential application of lipid nanoparticles as drug delivery systems for the retina and also as nonviral vectors in gene therapy of retina disorders, although much more effort is still needed before these lipidic systems could be available in the market.