Journal ArticleDOI
Understanding the mechanism of protamine in solid lipid nanoparticle-based lipofection: The importance of the entry pathway
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TLDR
In spite of the high DNA condensation capacity of protamine and its content in NLS, this does not always lead to an improvement in cell transfection since it may impair some of the limiting steps of the transfections processes.About:
This article is published in European Journal of Pharmaceutics and Biopharmaceutics.The article was published on 2011-11-01. It has received 88 citations till now. The article focuses on the topics: Protamine & Transfection.read more
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Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
Joanna Rejman,Massimo Conese +1 more
TL;DR: In this article, the authors investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine and K(+) depletion) and of caveolae-mediated uptake (filipin and genistein) on internalization of FITC-poly-l-lysine-labeled DOTAP/DNA lipoplexes and PEI/DNA polyplexes by A549 pneumocytes and HeLa cells and on the transfection efficiencies of these complexes with the luciferase gene.
Journal ArticleDOI
Nanotherapy for posterior eye diseases
Indu Pal Kaur,Shilpa Kakkar +1 more
TL;DR: In this paper, the authors discuss various pathways available for effective delivery to and clearance from the posterior eye, viz. liposomes, nanoparticles, and nanoemulsion, with illustrative examples, tables and flowcharts.
Journal ArticleDOI
Transferrin receptor specific nanocarriers conjugated with functional 7peptide for oral drug delivery.
Wenwen Du,Yuchen Fan,Nan Zheng,Bing He,Lan Yuan,Hua Zhang,Xueqing Wang,Jiancheng Wang,Xuan Zhang,Qiang Zhang +9 more
TL;DR: The functional nanocarriers could specifically interact with gastrointestinal endothelial cells, increase their transport and alter their pathway as a result.
Journal ArticleDOI
A novel cationic niosome formulation for gene delivery to the retina
Gustavo Puras,Mohamed Mashal,Jon Zarate,Mireia Agirre,Edilberto Ojeda,Santiago Grijalvo,Ramon Eritja,Ariadna Díaz-Tahoces,G. Martínez Navarrete,Marcelino Avilés-Trigueros,Eduardo Fernández,José Luis Pedraz +11 more
TL;DR: A novel niosome formulation based on the 2,3-di(tetradecyloxy)propan-1-amine cationic lipid combined with squalene and polysorbate 80 was elaborated to evaluate the transfection efficiency in rat retinas and successfully transfected HEK-293 and specially ARPE-19 cells, without affecting the viability.
Journal ArticleDOI
Lipid nanoparticles (SLN, NLC): Overcoming the anatomical and physiological barriers of the eye - Part II - Ocular drug-loaded lipid nanoparticles.
TL;DR: The latest findings, potential applications, and challenges related to the use of lipid nanoparticles for ocular drug delivery are comprehensively discussed.
References
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Journal ArticleDOI
Endocytosis of Nanomedicines
TL;DR: This review describes the current experimental tools to study endocytosis of nanomedicines and provides specific examples from recent literature and the authors' own work on endocyTosis of Nanomedicine.
Journal ArticleDOI
Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
TL;DR: Fluorescence colocalization studies with a lysosomal marker, AlexaFluor-dextran, revealed that polyplexes taken up by clathrin-mediated endocytosis are targeted to the lysOSomal compartment for degradation, while the polyplexed via caveolae escape this compartment, permitting efficient transfection.
Journal Article
Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
Joanna Rejman,Massimo Conese +1 more
TL;DR: In this article, the authors investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine and K(+) depletion) and of caveolae-mediated uptake (filipin and genistein) on internalization of FITC-poly-l-lysine-labeled DOTAP/DNA lipoplexes and PEI/DNA polyplexes by A549 pneumocytes and HeLa cells and on the transfection efficiencies of these complexes with the luciferase gene.
Journal ArticleDOI
Cationic lipids, lipoplexes and intracellular delivery of genes
Luc Wasungu,Dick Hoekstra +1 more
TL;DR: Some recent developments in the field on the structure/function relationship of cationic lipids in the mechanism of transfection and a comparison with mechanisms of viral and polyplex-mediated gene delivery are discussed.
Journal ArticleDOI
The Internalization Route Resulting in Successful Gene Expression Depends on both Cell Line and Polyethylenimine Polyplex Type
Katharina von Gersdorff,Niek N. Sanders,Roosmarijn E. Vandenbroucke,Stefaan C. De Smedt,Ernst Wagner,Manfred Ogris +5 more
TL;DR: The study revealed that the concentration window between specific inhibitory function and nonspecific toxicity of the uptake inhibitors was very narrow, and the internalization pathway mediating successful transfection depended on both cell type and polyplex type applied.