Ana J. García-Sáez

TL;DR: Tube scanning fluorescence cross-correlation spectroscopy is introduced for the absolute quantification of diffusion and complex formation of fluorescently labeled molecules in the mitochondrial compartments and it is discovered that practically all mitochondria-bound Bcl-xL and tBid are associated with each other, in contrast to undetectable association in the cytosol.

TL;DR: Rugby protocols to investigate the effect of pore-forming proteins in supported lipid bilayers are described and both the structural reorganization of the membrane surface and the changes in the force required for membrane piercing upon incubation with this special type of proteins are described.

TL;DR: The theoretical basis of point FCS as well as the scanning FCS (SFCS) approach are described, which is a practical way to address the challenges of FCS with membranes.

TL;DR: The findings reconcile apparent contradictions about the essential role of different ions in necroptosis taking into account that different ions could pass through punched membranes, and demonstrate that early calcium influx does not modulate the size of the later pores formed in the plasma membrane.

TL;DR: An artificial cytotoxic synapse is developed to examine how mobility and geometry of an anti‐CD95 agonistic antibody affect receptor aggregation and mobility, ie the first step of receptor activation.