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Ana J. García-Sáez
Researcher at University of Cologne
Publications - 131
Citations - 10109
Ana J. García-Sáez is an academic researcher from University of Cologne. The author has contributed to research in topics: Membrane & Vesicle. The author has an hindex of 37, co-authored 115 publications receiving 6333 citations. Previous affiliations of Ana J. García-Sáez include Max Planck Society & University of Tübingen.
Papers
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Book ChapterDOI
Analysis of Membrane‐Protein Complexes by Single‐Molecule Methods
Katia Cosentino,Katia Cosentino,Stephanie Bleicken,Stephanie Bleicken,Ana J. García-Sáez,Ana J. García-Sáez +5 more
Journal ArticleDOI
Lipids glue BAK dimers together.
TL;DR: A recent structure of the core domain of active BAK dimers with bound phospholipid molecules reveals a new bridging mechanism by which lipids drive BAX and BAK oligomerization and membrane pore formation.
Posted ContentDOI
Systematic assessment of the accuracy of subunit counting in biomolecular complexes using automated single molecule brightness analysis
John S. H. Danial,Yuri Quintana,Uris Ros,Raed Shalaby,Eleonora Germana Margheritis,Sabrina Chumpen,Christian Ungermann,Ana J. García-Sáez,Katia Cosentino +8 more
TL;DR: In this article, the authors developed a high-throughput, automated computational pipeline for single molecule brightness analysis that requires minimal human input, and used this strategy to systematically quantify the accuracy of counting under a wide range of experimental conditions.
Reference EntryDOI
Membrane Dynamics: Fluorescence Spectroscopy
TL;DR: In this article, the authors introduce four pioneer fluorescence-based approaches that have substantially broadened our overall understanding of membranes: fluorescence correlation spectroscopy (FCS), single particle tracking (SPT), fluorescence recovery after photobleaching (FRAP), and 6-lauroyl-2-(dimethylamino)-naphtalene (LAURDAN) microscopy.
Book ChapterDOI
Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions
TL;DR: This chapter describes the application of fluorescence correlation spectroscopy (FCS) as a powerful technique for the study of membrane organization and interactions.