Andreas Tauch

TL;DR: Small mobilizable vectors based on the Escherichia coli plasmids pK18 and pK19 can be transferred by RP4-mediated conjugation into a wide range of Gram- and Gram+ bacteria, and should facilitate gene disruption and allelic exchange by homologous recombination.

TL;DR: An account of the recent explosion of actinobacterial genomics data is provided and an attempt to place this in a biological and evolutionary context.

TL;DR: These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil.

TL;DR: Results suggest that species related to those of the genus Methanoculleus play a dominant role in methanogenesis in the analysed fermentation sample, and assignment of numerous contig sequences toClostridial genomes including gene regions for cellulolytic functions indicates that clostridia are important for hydrolysis of cellulosic plant biomass in the biogas fermenter under study.

TL;DR: The objectives of this study are to summarize the published literature on tools for genetic engineering in C. glutamicum and to focus on new sophisticated and highly efficient methods in the fields of DNA transfer techniques, cloning vectors, integrative genetic tools, and antibiotic-free self-cloning.