A
Andrew Vorobyov
Researcher at University of Toronto
Publications - 3
Citations - 461
Andrew Vorobyov is an academic researcher from University of Toronto. The author has contributed to research in topics: Gene targeting & Cas9. The author has an hindex of 3, co-authored 3 publications receiving 354 citations.
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Journal ArticleDOI
A strategy for modulation of enzymes in the ubiquitin system.
Andreas Ernst,George V. Avvakumov,Jiefei Tong,Yihui Fan,Yanling Zhao,Philipp Alberts,Avinash Persaud,John R. Walker,Ana-Mirela Neculai,Dante Neculai,Andrew Vorobyov,Pankaj Garg,Linda G. Beatty,Pak-Kei Chan,Yu Chi Juang,Marie-Claude Landry,Christina Yeh,Christina Yeh,Elton Zeqiraj,Konstantina Karamboulas,Abdellah Allali-Hassani,Masoud Vedadi,Mike Tyers,Mike Tyers,Jason Moffat,Frank Sicheri,Frank Sicheri,Laurence Pelletier,Laurence Pelletier,Daniel Durocher,Daniel Durocher,Brian Raught,Daniela Rotin,Jianhua Yang,Michael Moran,Sirano Dhe-Paganon,Sirano Dhe-Paganon,Sachdev S. Sidhu +37 more
TL;DR: This work used massively diverse combinatorial libraries of ubiquitin variants to develop inhibitors of four deubiquitinases (DUBs) and analyzed the DUB-inhibitor complexes with crystallography to report a method to target the myriad enzymes that govern ubiquitination of protein substrates.
Journal ArticleDOI
Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR-Cas9 genome-editing efficiency.
Marella D. Canny,Nathalie Moatti,Leo C. K. Wan,Leo C. K. Wan,Amélie Fradet-Turcotte,Amélie Fradet-Turcotte,Danielle Krasner,Pedro A. Mateos-Gómez,Michal Zimmermann,Alexandre Orthwein,Alexandre Orthwein,Yu-Chi Juang,Wei Zhang,Sylvie M. Noordermeer,Eduardo Seclén,Marcus D. Wilson,Andrew Vorobyov,Meagan Munro,Andreas Ernst,Andreas Ernst,Timothy F. Ng,Timothy F. Ng,Tiffany Cho,Tiffany Cho,Paula M. Cannon,Sachdev S. Sidhu,Frank Sicheri,Frank Sicheri,Daniel Durocher,Daniel Durocher +29 more
TL;DR: expression of one variant, named i53 (inhibitor of 53BP1), in human and mouse cells, blocked accumulation of 53 BP1 at sites of DNA damage and improved gene targeting and chromosomal gene conversion with either double-stranded DNA or single-strander oligonucleotide donors by up to 5.6-fold.
Posted ContentDOI
A genetically encoded inhibitor of 53BP1 to stimulate homology-based gene editing
Canny,Leo C. K. Wan,Amélie Fradet-Turcotte,Alexandre Orthwein,N Moatti,Yu Chi Juang,Weilian Zhang,Sylvie M. Noordermeer,Wilson,Andrew Vorobyov,Meagan Munro,Andreas Ernst,M Zimmermann,TF Ng,Sachdev S. Sidhu,F Sicheri,Daniel Durocher +16 more
TL;DR: i53 is a potent selective inhibitor of 53BP1 and enhances gene targeting and chromosomal gene conversion, two HR-dependent reactions, and is a robust tool to enhance precise genome editing by canonical HR pathways.