Showing papers by "Angelika Amon published in 2004"
TL;DR: The functions of Cdc14 are reviewed and how this phosphatase is regulated to accomplish the coupling of mitotic processes.
Abstract: ▪ Abstract Completion of the cell cycle requires the temporal and spatial coordination of chromosome segregation with mitotic spindle disassembly and cytokinesis. In budding yeast, the protein phosphatase Cdc14 is a key regulator of these late mitotic events. Here, we review the functions of Cdc14 and how this phosphatase is regulated to accomplish the coupling of mitotic processes. We also discuss the function and regulation of Cdc14 in other eukaryotes, emphasizing conserved features.
449 citations
TL;DR: Recent findings on how cell-cycle controls ensure the coordination of meiotic events, with a particular focus on the segregation of chromosomes are reviewed.
Abstract: Meiosis is the type of cell division that gives rise to eggs and sperm. Errors in the execution of this process can result in the generation of aneuploid gametes, which are associated with birth defects and infertility in humans. Here, we review recent findings on how cell-cycle controls ensure the coordination of meiotic events, with a particular focus on the segregation of chromosomes.
303 citations
TL;DR: It is proposed that Cdc14 released by the FEAR network mediates the partitioning of rDNA by facilitating the localization of condensin thereto and ensures that exit from mitosis is coupled to the completion of chromosome segregation.
294 citations
TL;DR: The screen described here provides a comprehensive analysis of the genes required for the meiotic cell cycle and identifies three factors important for the stepwise loss of sister chromatid cohesion.
Abstract: During meiosis, two chromosome segregation phases follow a single round of DNA replication We identified factors required to establish this specialized cell cycle by examining meiotic chromosome segregation in a collection of yeast strains lacking all nonessential genes This analysis revealed Sgo1, Chl4, and Iml3 to be important for retaining centromeric cohesin until the onset of anaphase II Consistent with this role, Sgo1 localizes to centromeric regions but dissociates at the onset of anaphase II The screen described here provides a comprehensive analysis of the genes required for the meiotic cell cycle and identifies three factors important for the stepwise loss of sister chromatid cohesion
273 citations
TL;DR: In budding yeast, a signaling network known as the Cdc fourteen early anaphase release (FEAR) network and its effector, the protein phosphatase Cdc14, play a key role in the coordination of the multiple events that occur during anaphases, such as partitioning of the DNA, regulation of spindle stability, activation of microtubule forces, and initiation of mitotic exit.
Abstract: Anaphase is the stage of the cell cycle when the duplicated genome is separated to opposite poles of the cell. The irreversible nature of this event confers a unique burden on the cell and it is therefore not surprising that the regulation of this cell cycle stage is complex. In budding yeast, a signaling network known as the Cdc fourteen early anaphase release (FEAR) network and its effector, the protein phosphatase Cdc14, play a key role in the coordination of the multiple events that occur during anaphase, such as partitioning of the DNA, regulation of spindle stability, activation of microtubule forces, and initiation of mitotic exit. These functions of the FEAR network contribute to genomic stability by coordinating the completion of anaphase and the execution of mitotic exit.
161 citations
TL;DR: The results indicate that Spo13 has the ability to regulate both the stepwise loss of sister chromatid cohesion and kinetochore coorientation, two essential features of meiotic chromosome segregation.
89 citations
TL;DR: Fob1 functions as a negative regulator of the FEAR network and is identified as a Spo12-interacting factor that helps to prevent the dissociation of Cdc14 from Cfi1/Net1 prior to anaphase and that Spo12 activation during earlyAnaphase promotes the release of CDC14 from its inhibitor by antagonizing Fob1 function.
63 citations
TL;DR: These findings suggest that late mitotic events are coupled by employing one pathway to control multiple events and that the spatial co-ordination of lateMitotic events seems also to be important in higher eukaryotes.
49 citations
TL;DR: GermOnline provides information and microarray expression data for genes involved in mitosis and meiosis, gamete formation and germ line development across species, as well as curated information on approximately 700 genes from various organisms.
Abstract: GermOnline provides information and microarray expression data for genes involved in mitosis and meiosis, gamete formation and germ line development across species. The database has been developed, and is being curated and updated, by life scientists in cooperation with bioinformaticists. Information is contributed through an online form using free text, images and the controlled vocabulary developed by the GeneOntology Consortium. Authors provide up to three references in support of their contribution. The database is governed by an international board of scientists to ensure a standardized data format and the highest quality of GermOnline's information content. Release 2.0 provides exclusive access to microarray expression data from Saccharomyces cerevisiae and Rattus norvegicus, as well as curated information on approximately 700 genes from various organisms. The locus report pages include links to external databases that contain relevant annotation, microarray expression and proteome data. Conversely, the Saccharomyces Genome Database (SGD), S.cerevisiae GeneDB and Swiss-Prot link to the budding yeast section of GermOnline from their respective locus pages. GermOnline, a fully operational prototype subject-oriented knowledgebase designed for community annotation and array data visualization, is accessible at http://www.germonline.org. The target audience includes researchers who work on mitotic cell division, meiosis, gametogenesis, germ line development, human reproductive health and comparative genomics.
29 citations