Anna Sherwood

TL;DR: A multiplex PCR with a mixture of primers targeting the rearranged variable and joining segments to capture receptor diversity is applied to a multiplex T cell receptor gamma sequencing assay and can be extended to any adaptive immune locus.

TL;DR: Next-generation sequencing of lymphoid receptor gene repertoire may improve clinical diagnosis and subsequent MRD monitoring of lymphoproliferative disorders and lower the threshold of detection for MRD and affect treatment decisions.

TL;DR: A high-throughput method to pair TCR α and β segments without the need for single-cell technologies is reported and validated, which can leverage the diversity of TCR sequences to accurately pair hundreds of thousands of TCRA and TCRB sequences in a single experiment.

TL;DR: It is determined that the immune response in the tumor is different than in the adjacent mucosal tissue, and the number of shared clones is not dependent on distance between the samples, implying that CRC tumors induce a specific adaptive immune response, but that this response differs widely in strength and breadth between patients.

TL;DR: It is concluded that the cellular adaptive immune response within ovarian carcinomas is spatially homogeneous and distinct from the T‐cell compartment of peripheral blood.