Harlan Robins

TL;DR: It is shown that pre-existing CD8+ T cells distinctly located at the invasive tumour margin are associated with expression of the PD-1/PD-L1 immune inhibitory axis and may predict response to therapy.

TL;DR: A model is proposed whereby SNP309 serves as a rate-limiting event in carcinogenesis, and a single nucleotide polymorphism (SNP309) is found in the MDM2 promoter and is shown to increase the affinity of the transcriptional activator Sp1, resulting in higher levels ofMDM2 RNA and protein and the subsequent attenuation of the p53 pathway.

TL;DR: A novel experimental and computational approach is developed to measure TCR CDR3 diversity based on single-molecule DNA sequencing, and it is found that total TCRbeta receptor diversity is at least 4-fold higher than previous estimates, and the diversity in the subset of CD45RO(+) antigen-experienced alphabeta T cells is at at least 10-foldHigher than previously estimates.

TL;DR: A multiplex PCR with a mixture of primers targeting the rearranged variable and joining segments to capture receptor diversity is applied to a multiplex T cell receptor gamma sequencing assay and can be extended to any adaptive immune locus.

TL;DR: The authors show that the T cell repertoire is limited and biased from the time of the original V-D-J rearrangement during cell development, and this may help to understand these illnesses and to look for ways to modify patients’ T cell repertoires.