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Anne Arens
Researcher at German Cancer Research Center
Publications - 16
Citations - 1787
Anne Arens is an academic researcher from German Cancer Research Center. The author has contributed to research in topics: Gene & Genome. The author has an hindex of 12, co-authored 16 publications receiving 1671 citations. Previous affiliations of Anne Arens include Vita-Salute San Raffaele University.
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Journal ArticleDOI
An unbiased genome-wide analysis of zinc-finger nuclease specificity
Richard Gabriel,Angelo Lombardo,Anne Arens,Jeffrey C. Miller,Pietro Genovese,Christine Kaeppel,Ali Nowrouzi,Cynthia C. Bartholomae,Jianbin Wang,Geoffrey Friedman,Michael C. Holmes,Philip D. Gregory,Hanno Glimm,Manfred Schmidt,Luigi Naldini,Christof von Kalle +15 more
TL;DR: Comprehensive mapping of ZFN activity in vivo will facilitate the broad application of these reagents in translational research, and show that nonhomologous end-joining captures integrase-defective lentiviral vectors at DSBs, tagging these transient events.
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Distinct types of tumor-initiating cells form human colon cancer tumors and metastases
Sebastian M. Dieter,Claudia R. Ball,Christopher M. Hoffmann,Ali Nowrouzi,Friederike Herbst,Oksana Zavidij,Ulrich Abel,Ulrich Abel,Anne Arens,Wilko Weichert,Karsten Brand,Moritz Koch,Jürgen Weitz,Manfred Schmidt,Christof von Kalle,Hanno Glimm +15 more
TL;DR: It is demonstrated that tumor initiation, self-renewal, and metastasis formation are limited to particular subpopulations of TICs in primary human colon cancer.
Journal ArticleDOI
Comprehensive genomic access to vector integration in clinical gene therapy
Richard Gabriel,R Eckenberg,Anna Paruzynski,Cynthia C. Bartholomae,Ali Nowrouzi,Anne Arens,Steven J. Howe,Alessandra Recchia,Claudia Cattoglio,Wei Wang,Katrin Faber,Kerstin Schwarzwaelder,Romy Kirsten,Annette Deichmann,Claudia R. Ball,Kamaljit S. Balaggan,Rafael J. Yáñez-Muñoz,Robin R. Ali,H. Bobby Gaspar,Luca Biasco,Alessandro Aiuti,Daniela Cesana,Eugenio Montini,Luigi Naldini,Odile Cohen-Haguenauer,Fulvio Mavilio,Adrian J. Thrasher,Adrian J. Thrasher,Hanno Glimm,Christof von Kalle,William Saurin,Manfred Schmidt +31 more
TL;DR: A model to define genomic access to the viral integration site that provides optimal restriction motif combinations and minimizes the percentage of nonaccessible insertion loci is developed and a new nonrestrictive LAM-PCR approach is introduced.
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Genome-wide high-throughput integrome analyses by nrLAM-PCR and next-generation sequencing.
Anna Paruzynski,Anne Arens,Richard Gabriel,Cynthia C. Bartholomae,Simone Scholz,Wei Wang,Stephan Wolf,Hanno Glimm,Manfred Schmidt,Christof von Kalle +9 more
TL;DR: A step-by-step protocol for universal genome-wide and comprehensive integrome analysis that can be performed on >102–103 samples of interest in parallel and can be adapted to any PCR-based method aiming to characterize unknown flanking DNA adjacent to a known DNA region is reported.
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Hepatocyte-targeted expression by integrase-defective lentiviral vectors induces antigen-specific tolerance in mice with low genotoxic risk
Janka Matrai,Alessio Cantore,Cynthia C. Bartholomae,Andrea Annoni,Wei Wang,Abel Acosta-Sanchez,Ermira Samara-Kuko,Liesbeth De Waele,Ling Ma,Pietro Genovese,Martina Damo,Anne Arens,Kevin Goudy,Timothy C. Nichols,Christof von Kalle,Marinee Chuah,Maria Grazia Roncarolo,Manfred G. Schmidt,Thierry VandenDriessche,Luigi Naldini +19 more
TL;DR: Hepatocyte‐targeted expression using IDLVs resulted in the sustained and robust induction of immune tolerance to both intracellular and secreted proteins, despite the reduced transgene expression levels in comparison with their integrase‐competent vector counterparts.