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Annemie Jacobs
Researcher at Vrije Universiteit Brussel
Publications - 5
Citations - 126
Annemie Jacobs is an academic researcher from Vrije Universiteit Brussel. The author has contributed to research in topics: Allosteric regulation & Aspartate carbamoyltransferase. The author has an hindex of 5, co-authored 5 publications receiving 126 citations. Previous affiliations of Annemie Jacobs include VU University Amsterdam.
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Journal ArticleDOI
Heterotropic interactions in aspartate transcarbamoylase: turning allosteric ATP activation into inhibition as a consequence of a single tyrosine to phenylalanine mutation.
F. Van Vliet,Xu-Guang Xi,C. De Staercke,B. De Wannemaeker,Annemie Jacobs,J. Cherfils,Moncef M. Ladjimi,G. Herve,Raymond Cunin +8 more
TL;DR: The result shows that the hydrophobic interface between the two domains of the regulatory chain plays an important role in the discrimination between the regulatory signals promoted by the two allosteric effectors.
Journal ArticleDOI
Positive and negative regulation of CAR1 Expression in Saccharomyces cerevisiae
Raymond Cunin,Evelyne Dubois,Gerrit Vanthienen,Kristof Tinel,Annemie Jacobs,Marjolaine Crabeel +5 more
TL;DR: Fusion tolacZ of several fragments of the 5′ non-coding region showed that induction ofCAR1 by arginine is positively regulated by the products of theARGR genes, and the order of the regulatory regions was confirmed: 5′—nitrogen catabolite repression—activation byArginine—CARGRI-mediated repression—CAR1.
Journal ArticleDOI
Intramolecular transmission of the ATP regulatory signal in Escherichia coli aspartate transcarbamylase: specific involvement of a clustered set of amino acid interactions at an interface between regulatory and catalytic subunits.
Christine De Staercke,Françoise Van Vliet,Xu-Guang Xi,Christiana Swarupa Rani,Moncef M. Ladjimi,Annemie Jacobs,Françoise Triniolles,Guy Hervé,Raymond Cunin +8 more
TL;DR: The identification of a cluster of amino acid interactions at an interface between the regulatory chains and the catalytic chains of the enzyme as another structural feature involved in the transmission of the ATP regulatory signal but not in those of CTP and UTP is described.
Journal ArticleDOI
The Activation of Escherichia coli Aspartate Transcarbamylase by ATP: Specific Involvement of Helix H2′ at the Hydrophobic Interface Between the Two Domains of the Regulatory Chains
Xu-Guang Xi,Christine De Staercke,Françoise Van Vliet,Françoise Triniolles,Annemie Jacobs,Philippe P. Stas,Moncef M. Ladjimi,Valérie Simon,Raymond Cunin,Guy Hervé +9 more
TL;DR: The properties of the mutants and the results of modelling suggest that a movement of helix H2' is part of the mechanism of activation by ATP, and a model is proposed to account for the transmission of the ATP signal from the regulatory site to the interface between the regulatory and catalytic chains.
Journal ArticleDOI
Structure-function relationship in allosteric aspartate carbamoyltransferase from Escherichia coli. I. Primary structure of a pyrI gene encoding a modified regulatory subunit.
Raymond Cunin,Annemie Jacobs,Daniel Charlier,Marjolaine Crabeel,Guy Hervé,Nicolas Glansdorff,André Piérard +6 more
TL;DR: The sequence of the mutated pyrI gene is reported and it is shown that, during the generation of this pyrBI-bearing phage, six codons from lambda DNA have been substituted for the eight terminal codons of the wild-type gene.