Anthony M. Paradiso

TL;DR: expression of the human P2U receptor (HP2U) in 1321N1 cells revealed evidence for autocrine ATP release and stimulation of transduced receptors, which will be useful for studying autocrine regulatory mechanisms and in screening of potential therapeutic drugs.

TL;DR: How the normal coordination of opposing ion transport pathways to maintain PCL volume is disrupted in cystic fibrosis is demonstrated, the hitherto unknown role of phasic motion in regulating key aspects of normal and CF innate airways defense is discovered, and maneuvers directed at increasing motion-induced nucleotide release may be therapeutic in CF patients are demonstrated.

TL;DR: If performed properly, the in vivo nasal PD technique clearly discriminates between normal subjects and cystic fibrosis patients, and can yield estimates of the biological efficacy of gene transfer to achieve correction of the electrolyte transport defects in CF patients.

TL;DR: C cultured human CF bronchial epithelial pHASL is abnormally regulated under basal conditions because of absent CFTR-dependent defect, and this defect can lead to an impaired capacity to respond to airway conditions associated with acidification of ASL.

TL;DR: It is concluded that HPV-18 E6 and E7 genes are sufficient to transform human airway epithelial cells and that the resultant cell lines express differentiated phenotypic properties that approximate those of the native epithelium.