Anthony Squire

TL;DR: Fluorescence lifetime imaging microscopy is a technique in which the mean fluorescence lifetime of a chromophore is measured at each spatially resolvable element of a microscope image to allow exploration of the molecular environment of labelled macromolecules in the interior of cells.

TL;DR: Most of the RTKs activated at the cell surface showed interaction with PTP1B after internalization, establishing that RTK activation and inactivation are spatially and temporally partitioned within cells.

TL;DR: Protein kinase C (PKC) has been implicated in integrin‐mediated spreading and migration in mammary epithelial cells there is a partial co‐localization between β1 integrin and PKCα and this PKC α‐enhanced migratory response is inhibited by blockade of endocytosis.

TL;DR: Spatially resolved fluorescence resonance energy transfer measured by fluorescence lifetime imaging microscopy (FLIM), provides a method for tracing the catalytic activity of fluorescently tagged proteins inside live cell cultures and enables determination of the functional state of proteins in fixed cells and tissues.

TL;DR: Global analysis techniques are described for frequency domain fluorescence lifetime imaging microscopy (FLIM) data that exploit the prior knowledge that only a limited number of fluorescent molecule species whose lifetimes do not vary spatially are present.