Global analysis of fluorescence lifetime imaging microscopy data.
Reads0
Chats0
TLDR
Global analysis techniques are described for frequency domain fluorescence lifetime imaging microscopy (FLIM) data that exploit the prior knowledge that only a limited number of fluorescent molecule species whose lifetimes do not vary spatially are present.About:
This article is published in Biophysical Journal.The article was published on 2000-04-01 and is currently open access. It has received 238 citations till now. The article focuses on the topics: Fluorescence-lifetime imaging microscopy.read more
Citations
More filters
Journal ArticleDOI
The Phasor Approach to Fluorescence Lifetime Imaging Analysis
TL;DR: The phasor approach has the potential to simplify the way data are analyzed in FLIM, paving the way for the analysis of large data sets and, in general, making the FLIM technique accessible to the nonexpert in spectroscopy and data analysis.
Journal ArticleDOI
Reliable and Global Measurement of Fluorescence Resonance Energy Transfer Using Fluorescence Microscopes
Zongping Xia,Yuechueng Liu +1 more
TL;DR: A modified method for FRET quantification in cultured cells using conventional fluorescence microscopy was presented, which gave consistent FRET values that could be compared among different cells with varying protein expression levels.
Journal ArticleDOI
Spectral imaging and its applications in live cell microscopy
TL;DR: Recent progress on the technical implementation ofSpectral imaging and linear unmixing extends the possibilities to discriminate distinct fluorophores with highly overlapping emission spectra and thus the possibilities of multicolor imaging.
Journal ArticleDOI
Imaging biochemistry inside cells
TL;DR: The development and use of sensors based on the genetically encoded variants of green-fluorescent proteins has facilitated the observation of 'live' biochemistry on a microscopic level, with the advantage of preserving the cellular context of biochemical connectivity, compartmentalization and spatial organization.
Journal ArticleDOI
Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue
Chiara Stringari,Amanda Cinquin,Olivier Cinquin,Michelle A. Digman,Peter J. Donovan,Enrico Gratton +5 more
TL;DR: The cell phasor approach to lifetime imaging provides a label- free, fit-free, and sensitive method to identify different metabolic states of cells during differentiation, to sense small changes in the redox state of cells, and may identify symmetric and asymmetric divisions and predict cell fate.
References
More filters
Numerical recipes in C
TL;DR: The Diskette v 2.06, 3.5''[1.44M] for IBM PC, PS/2 and compatibles [DOS] Reference Record created on 2004-09-07, modified on 2016-08-08.
BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
The differentiation of pseudoinverses and nonlinear least squares problems whose variables separate.
Gene H. Golub,Victor Pereyra +1 more
TL;DR: Algorithms are presented which make extensive use of well-known reliable linear least squares techniques, and numerical results and comparisons are given.
The differentiation of pseudo-inverses and non-linear least squares problems whose variables separate.
G. H. GOLUBf,V. Pereyra +1 more
TL;DR: In this paper, the least square fit of nonlinear models of the form {(0t, Yi), l,, m, qgj, ti, and the modified functional r2( 0t (lY O(0 t)/(0)yl)22) is considered.