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Anthony Squire
Researcher at Lincoln's Inn
Publications - 19
Citations - 2915
Anthony Squire is an academic researcher from Lincoln's Inn. The author has contributed to research in topics: Microscopy & Fluorescence-lifetime imaging microscopy. The author has an hindex of 14, co-authored 15 publications receiving 2811 citations.
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Journal ArticleDOI
Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell.
TL;DR: Fluorescence lifetime imaging microscopy is a technique in which the mean fluorescence lifetime of a chromophore is measured at each spatially resolvable element of a microscope image to allow exploration of the molecular environment of labelled macromolecules in the interior of cells.
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Imaging Sites of Receptor Dephosphorylation by PTP1B on the Surface of the Endoplasmic Reticulum
TL;DR: Most of the RTKs activated at the cell surface showed interaction with PTP1B after internalization, establishing that RTK activation and inactivation are spatially and temporally partitioned within cells.
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PKCα regulates β1 integrin‐dependent cell motility through association and control of integrin traffic
Tony Ng,David Shima,Anthony Squire,Philippe I. H. Bastiaens,Steve Gschmeissner,Martin J. Humphries,Peter J. Parker +6 more
TL;DR: Protein kinase C (PKC) has been implicated in integrin‐mediated spreading and migration in mammary epithelial cells there is a partial co‐localization between β1 integrin and PKCα and this PKC α‐enhanced migratory response is inhibited by blockade of endocytosis.
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Imaging Protein Kinase Cα Activation in Cells
Tony Ng,Anthony Squire,Gurdip Hansra,Frédéric Bornancin,Corinne Prevostel,Andrew M. Hanby,William H. Harris,Diana M. Barnes,Sandra Schmidt,Harry Mellor,Philippe I. H. Bastiaens,Peter J. Parker +11 more
TL;DR: Spatially resolved fluorescence resonance energy transfer measured by fluorescence lifetime imaging microscopy (FLIM), provides a method for tracing the catalytic activity of fluorescently tagged proteins inside live cell cultures and enables determination of the functional state of proteins in fixed cells and tissues.
Journal ArticleDOI
Global analysis of fluorescence lifetime imaging microscopy data.
TL;DR: Global analysis techniques are described for frequency domain fluorescence lifetime imaging microscopy (FLIM) data that exploit the prior knowledge that only a limited number of fluorescent molecule species whose lifetimes do not vary spatially are present.