Anwei Hou

TL;DR: It is demonstrated that the promiscuous terpene synthases can produce more variable terpenoids in vivo by converting precursor polyisoprenoid diphosphates of different lengths and integrated the engineered MVA pathway, combinatorial biosynthesis, and point mutagenesis to depict the comprehensive product profiles.

TL;DR: Based on a terpenoid overproduction platform in yeast for genome mining, a chimeric diterpene synthase from the endophytic fungus Colletotrichum gloeosporioides ES026 was characterized as the (5R,12R,14S)-dolasta-1(15),8-dienes synthase, establishing the predicted C1-III-IV cyclization mode for this first characterized clade II-D enzyme.

TL;DR: The potential power of sesquiterpene synthase FgJ03939 from Fusarium graminearum was fully exploited in a farnesyl diphosphate-overexpressing Saccharomyces cerevisiae chassis to produce the novel sesQUiterpenes fusariumdiene, epi-fusagramineol, and fusagraminol.

TL;DR: A biosynthetic gene cluster from Streptomyces mobaraensis encoding the first cases of a bacterial geranylfarnesyl diphosphate synthase and a type I sesterterpene synthase was identified and the structures of seven sestersterpenes produced by these enzymes were elucidated.

TL;DR: Substrate profile indicated that hydroxyethyl-3-oxo-4-(2,4,5-trifluorophenyl)butanoate showed the best biocatalytic performance, partially due to its best solubility in the enzymatic system.