Atsuo Waki

TL;DR: A 3D culture system with inorganic nanoscale scaffolding using nanoimprinting technology (nano-culture plates) allows creating uniform and highly-reproducible 3D cultures, which can be used for high-throughput/high-content screening of anticancer drugs and should accelerate discovery of more effective anticancer therapies.

TL;DR: This work investigated the relation between [14C]choline metabolism and proliferative activity using 10 tumor cell lines and fibroblasts and found that choline uptake was higher in tumor cells than in fibro Blasts and was correlated with the proliferativeActivity, though the sensitivity of [14 cCholine uptake to proliferation activity was less than that of [1-14C]-acetate.

TL;DR: It is found that tumor cells expressed higher levels of cytosolic acetyl‐CoA synthetase (ACSS2) under hypoxia than normoxia, which indicates that ACSS2 is a bi‐directional enzyme in tumor cells and that AC SS2 might play a buffering role in tumor acetyl-CoA/acetate metabolism.

TL;DR: It is demonstrated that uptake of radiolabeled acetate is a useful predictor of FASN-targeted therapy outcome, which suggests that [1-11C]acetate positron emission tomography (PET) could be a powerful tool to accomplish personalized FASn- targeted therapy by non-invasive visualization of tumor acetate uptake and selection of responsive tumors.

TL;DR: An innovative high-throughput screening system using nanoimprinting 3D culture to simulate in vivo conditions, thereby facilitating efficient drug development and developing drugs that effectively inhibit cancer growth in vivo as compared to 2D culture is reported.