Benjamin K. Law

TL;DR: In this paper, site-specific and multiple-site conjugation of a wide assortment of molecules on both the termini and internal sites of Cas9, creating a platform for endowing Cas9 with diverse functions, using this platform, Cas9 can be modified to more precisely incorporate exogenously supplied singlestranded oligonucleotide donor (ssODN) at the DNA break site.

TL;DR: By photocaging this Cas9 activator to render it biologically inert and photoactivatable, and employing next-generation protein engineering approaches, this work has built a system with a wide dynamic range, low background, and fast photoactivation using a low-intensity light while rendering the small-molecule activator biologically inert.

TL;DR: A chemogenetic system is developed that brings Cas9 in proximity to a ubiquitin ligase, enabling rapid ubiquitination and degradation of Cas9 by the proteasome, and is able to dosably control the Cas9 activity and specificity to ameliorate the off-target effects.

TL;DR: It is demonstrated that the multivalent display of ss ODN on Cas9 significantly increased precision genome edits over those of Cas9 bearing one or no ssODN, and such display platform is compatible with large oligonucleotides and rapid screening of ssODNs.