B
Brecht Crombez
Researcher at Ghent University Hospital
Publications - 4
Citations - 139
Brecht Crombez is an academic researcher from Ghent University Hospital. The author has contributed to research in topics: Germline mutation & Breast cancer. The author has an hindex of 4, co-authored 4 publications receiving 116 citations. Previous affiliations of Brecht Crombez include Ghent University.
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Journal ArticleDOI
Flexible, scalable, and efficient targeted resequencing on a benchtop sequencer for variant detection in clinical practice.
Kim De Leeneer,Jan Hellemans,Wouter Steyaert,Steve Lefever,Inge Vereecke,Eveline Debals,Brecht Crombez,Machteld Baetens,Mattias Van Heetvelde,Frauke Coppieters,Jo Vandesompele,Annelies De Jaegher,Elfride De Baere,Paul Coucke,Kathleen Claes +14 more
TL;DR: Although the singleplex PCR‐based enrichment platform was optimized for constitutional variant detection of monogenic disease genes, it is now also used as a model for somatic mutation detection in acquired diseases.
Journal ArticleDOI
BRCA1, BRCA2 and PALB2 mutations and CHEK2 c.1100delC in different South African ethnic groups diagnosed with premenopausal and/or triple negative breast cancer.
Flavia Zita Francies,Flavia Zita Francies,Tasha Wainstein,K De Leeneer,A. Cairns,Marshall Murdoch,Sarah Nietz,H. Cubasch,Bruce Poppe,T. Van Maerken,Brecht Crombez,Ilse Coene,Robyn Kerr,Jacobus Slabbert,Anne Vral,Amanda Krause,Ans Baeyens,Ans Baeyens,Ans Baeyens,Kathleen Claes +19 more
TL;DR: This NGS study shows that mutation analysis is warranted in South African patients with triple negative and/or in premenopausal breast cancer, and highlights the importance of evaluating germline mutations in major breast cancer genes in all of the South African population groups.
Journal ArticleDOI
Prevalence of BRCA1/2 mutations in sporadic breast/ovarian cancer patients and identification of a novel de novo BRCA1 mutation in a patient diagnosed with late onset breast and ovarian cancer: implications for genetic testing
Kim De Leeneer,Ilse Coene,Brecht Crombez,Justine Simkens,Rudy Van den Broecke,Alain Bols,Barbara Stragier,Ilse Vanhoutte,Anne De Paepe,Bruce Poppe,Kathleen Claes +10 more
TL;DR: The study shows that the mutation detection probability in specific patient subsets can be significant, therefore mutation analysis should be considered in sporadic patients, and a family history for the disease and an early age of onset should not be used as the only criteria for mutation analysis of BRCA1/2.
Journal ArticleDOI
Evaluation of relative quantification of alternatively spliced transcripts using droplet digital PCR
Mattias Van Heetvelde,Mattias Van Heetvelde,Wouter Van Loocke,Wim Trypsteen,Annelot Baert,Katrien Vanderheyden,Brecht Crombez,Jo Vandesompele,Kim De Leeneer,Kathleen Claes +9 more
TL;DR: The value of a highly optimized qPCR for the relative quantification of isoforms is shown, but cost efficiency and simplicity turned out to be better for RT-qPCR.