C. A. Pierson

TL;DR: The sterol profile of an ERG25 disruptant was consistent with the erg25 allele obtained by mutagenesis, and the amino acid sequence shows a C-terminal endoplasmic reticulum retrieval signal KKXX and three histidine-rich clusters found in eukaryotic membrane desaturases and in a bacterial alkane hydroxylase and xylene monooxygenase.

TL;DR: Gene disruption demonstrates that ERG5 is not essential for cell viability and the putative gene encoding the C-22 sterol desaturase required in ergosterol biosynthesis is identified.

TL;DR: Dap1p utilizes heme to stabilize Erg11p, which in turn regulates ergosterol synthesis and MMS resistance, suggesting that the Dap1P-cytochrome P450 protein pathway is broadly conserved in eukaryotic species.

TL;DR: The Saccharomyces cerevisiae ERG24 gene, encoding sterol delta 14 reductase (Erg24p), was cloned by selecting strains carrying sequences on a 2 mu-based vector for resistance to the morpholine fungicide, fenpropimorph (Fp).

TL;DR: It is demonstrated that treatment of the sterol auxotrophs erg25 slu1 or erg11 slu2 with azole antibiotics paradoxically restores viability to these strains in the absence of sterol supplementation via the suppression system described.