C
Caroline J. DeHart
Researcher at Northwestern University
Publications - 28
Citations - 1070
Caroline J. DeHart is an academic researcher from Northwestern University. The author has contributed to research in topics: Proteomics & Chemistry. The author has an hindex of 16, co-authored 24 publications receiving 692 citations. Previous affiliations of Caroline J. DeHart include Princeton University.
Papers
More filters
Journal ArticleDOI
Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.
Daniel P. Donnelly,Catherine M. Rawlins,Caroline J. DeHart,Luca Fornelli,Luis F. Schachner,Ziqing Lin,Jennifer L. Lippens,Krishna Aluri,Krishna Aluri,Richa Sarin,Richa Sarin,Bifan Chen,Carter Lantz,Wonhyeuk Jung,Kendall Johnson,Antonius Koller,Jeremy J. Wolff,Iain D. G. Campuzano,Jared R. Auclair,Alexander R. Ivanov,Julian P. Whitelegge,Ljiljana Paša-Tolić,Julia Chamot-Rooke,Paul O. Danis,Lloyd M. Smith,Yury O. Tsybin,Joseph A. Loo,Ying Ge,Neil L. Kelleher,Jeffrey N. Agar +29 more
TL;DR: The Consortium for Top-Down Proteomics presents a decision-tree-based guide to sample preparation and analysis protocols for researchers performing top-down mass-spectrometry-based analysis of intact proteins.
Journal ArticleDOI
Identification and Characterization of Human Proteoforms by Top-Down LC-21 Tesla FT-ICR Mass Spectrometry.
Lissa C. Anderson,Caroline J. DeHart,Nathan K. Kaiser,Ryan T. Fellers,Donald F. Smith,Joseph B. Greer,Richard D. LeDuc,Greg T. Blakney,Paul M. Thomas,Neil L. Kelleher,Christopher L. Hendrickson +10 more
TL;DR: Top-down LC-21 T FT-ICR MS/MS of intact proteins derived from human colorectal cancer cell lysate is demonstrated, identifying a combined total of 684 unique protein entries observed as 3238 unique proteoforms at a 1% false discovery rate.
Journal ArticleDOI
The biosynthesis of methanobactin.
Grace E. Kenney,Laura M. K. Dassama,Maria-Eirini Pandelia,Anthony S. Gizzi,Ryan J. Martinie,Peng Gao,Caroline J. DeHart,Luis F. Schachner,Owen S. Skinner,Soo Y. Ro,Xiao Zhu,Monica Sadek,Paul M. Thomas,Steven C. Almo,J. Martin Bollinger,Carsten Krebs,Neil L. Kelleher,Amy C. Rosenzweig +17 more
TL;DR: The core biosynthetic machinery responsible for the characteristic posttranslational modifications that grant methanobactin its specificity and affinity for copper are described.
Journal ArticleDOI
Expansion for the Brachylophosaurus canadensis Collagen I Sequence and Additional Evidence of the Preservation of Cretaceous Protein
Elena R. Schroeter,Caroline J. DeHart,Timothy P. Cleland,Wenxia Zheng,Paul M. Thomas,Neil L. Kelleher,Marshall Bern,Mary Higby Schweitzer,Mary Higby Schweitzer +8 more
TL;DR: The data robustly support the hypothesis of an endogenous origin for these peptides, confirm the idea that peptides can survive in specimens tens of millions of years old, and bolster the validity of the 2009 study.
Journal ArticleDOI
Precise characterization of KRAS4b proteoforms in human colorectal cells and tumors reveals mutation/modification cross-talk.
Ioanna Ntai,Luca Fornelli,Caroline J. DeHart,Josiah E. Hutton,Peter F. Doubleday,Richard D. LeDuc,Alexandra J. van Nispen,Ryan T. Fellers,Gordon Whiteley,Emily S. Boja,Henry Rodriguez,Neil L. Kelleher +11 more
TL;DR: An intact protein assay was developed for the detection and quantitation of KRAS protein forms (proteoforms), enabling the measurement of how genetically encoded mutations affect posttranslational modifications on the same protein molecule.