Christian Lanctôt

TL;DR: This work has shown that the dynamic nature of the positioning of genetic material in the nuclear space and the higher-order architecture of the nucleus are integrated is essential to the overall understanding of gene regulation.

TL;DR: It is shown that the nuclear architecture of rod photoreceptor cells differs fundamentally in nocturnal and diurnal mammals and suggests that the conventional architecture prevails in eukaryotic nuclei because it results in more flexible chromosome arrangements, facilitating positional regulation of nuclear functions.

TL;DR: This chapter provides protocols for the preparation of complex DNA-probe sets suitable for 3D-FISH with up to six different fluorochromes, for 3Ds on cultured mammalian cells (growing in suspension or adherently) as well as on tissue sections, and for3D immuno-Fish.

TL;DR: The role of the interchromatin compartment (IC) in shaping nuclear landscapes is focused on and it is postulated that it provides routes for imported transcription factors to target sites, for export routes of mRNA as ribonucleoproteins toward NPCs, as well as for the intranuclear passage of regulatory RNAs from sites of transcription to remote functional sites.

TL;DR: This work explores how causal relationships between genome activity and nuclear and large‐scale chromatin structure and how these might be uncovered and played an important role in the cell‐type specific orchestration of the expression of thousands of genes in eukaryotic cells.