C
Christine E. Bear
Researcher at University of Toronto
Publications - 166
Citations - 9000
Christine E. Bear is an academic researcher from University of Toronto. The author has contributed to research in topics: Cystic fibrosis transmembrane conductance regulator & Chloride channel. The author has an hindex of 44, co-authored 155 publications receiving 8240 citations. Previous affiliations of Christine E. Bear include Hospital for Sick Children.
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Journal ArticleDOI
Purification and functional reconstitution of the cystic fibrosis transmembrane conductance regulator (CFTR)
Christine E. Bear,Canhui Li,Norbert Kartner,R. J. Bridges,Tim J. Jensen,Mohabir Ramjeesingh,John R. Riordan +6 more
TL;DR: In this paper, a recombinant CFTR protein from a high-level baculovirus-infected insect cell line was purified to homogeneity, and the protein exhibited regulated chloride channel activity, providing evidence that the protein itself is the channel.
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Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance
Norbert Kartner,John W. Hanrahan,Tim J. Jensen,A. Leonard Naismith,Shi-Zhang Sun,Cameron Ackerley,Evangelica F. Reyes,Lap-Chee Tsui,Johanna M. Rommens,Christine E. Bear,John R. Riordan +10 more
TL;DR: It is demonstrated that CFTR can function in heterologous nonepithelial cells and lend support to the possibility thatCFTR may itself be a regulated anion channel.
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Modulation of disease severity in cystic fibrosis transmembrane conductance regulator deficient mice by a secondary genetic factor
Richard Rozmahel,M. Wilschanski,Angabin Matin,Suzanne Plyte,Mary Oliver,Wojtek Auerbach,Aideen M. Moore,Janet F. Forstner,Peter R. Durie,Joseph H. Nadeau,Christine E. Bear,Lap-Chee Tsui +11 more
TL;DR: Electrophysiological studies on mice with prolonged survival show that the partial rectification of Cl− and Na+ ion transport abnormalities can be explained in part by up-regulation of a calcium-activated Cl− conductance.
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The delta F508 mutation decreases the stability of cystic fibrosis transmembrane conductance regulator in the plasma membrane. Determination of functional half-lives on transfected cells.
Gergely L. Lukacs,Xiu Bao Chang,Christine E. Bear,Norbert Kartner,Abdalla J. Mohamed,John R. Riordan,Sergio Grinstein +6 more
TL;DR: The results indicate that the structural abnormality of delta F508CFTR affects not only the delivery of the protein to the plasma membrane, but also its stability therein, and suggest that overcoming the processing block at the endoplasmic reticulum may not suffice to restore normal Cl- conductance in CF.
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Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein.
Amy P. Wong,Christine E. Bear,Stephanie Chin,Peter Pasceri,Tadeo Thompson,Ling-Jun Huan,Felix Ratjen,James Ellis,Janet Rossant +8 more
TL;DR: This study provides a method for generating patient-specific airway epithelial cells for disease modeling and in vitro drug testing and reports an in vitro directed differentiation protocol for generating functional CFTR-expressingAirway epithelia from human embryonic stem cells.