C
Christopher A. Werley
Researcher at Harvard University
Publications - 25
Citations - 1803
Christopher A. Werley is an academic researcher from Harvard University. The author has contributed to research in topics: Optogenetics & Medicine. The author has an hindex of 13, co-authored 21 publications receiving 1516 citations. Previous affiliations of Christopher A. Werley include Massachusetts Institute of Technology & Howard Hughes Medical Institute.
Papers
More filters
Journal ArticleDOI
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Daniel Hochbaum,Yongxin Zhao,Samouil L. Farhi,Nathan C. Klapoetke,Christopher A. Werley,Vikrant Kapoor,Peng Zou,Joel M. Kralj,Dougal Maclaurin,Niklas Smedemark-Margulies,Jessica L. Saulnier,Gabriella L. Boulting,Christoph Straub,Yong Ku Cho,Michael Melkonian,Gane Ka-Shu Wong,D. Jed Harrison,Venkatesh N. Murthy,Bernardo L. Sabatini,Edward S. Boyden,Robert E. Campbell,Adam E. Cohen +21 more
TL;DR: In this article, a coexpression vector, Optopatch, enabled cross-talk-free genetically targeted all-optical electrophysiology without the use of conventional electrodes.
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Daniel Hochbaum,Yongxin Zhao,Samouil L. Farhi,Christopher A. Werley,Vikrant Kapoor,Peng Zou,Joel M. Kralj,Dougal Maclaurin,Niklas Smedemark-Margulies,Jessica L. Saulnier,Gabriella L. Boulting,Christoph Straub,Michael Melkonian,Gane Ka-Shu Wong,D. Jed Harrison,Venkatesh N. Murthy,Bernardo L. Sabatini,Robert E. Campbell,Adam E. Cohen,Nathan C. Klapoetke,Yong Ku Cho,Edward S. Boyden +21 more
TL;DR: The Optopatch platform enables high-throughput, spatially resolved electrophysiology without the use of conventional electrodes and revealed homeostatic tuning of intrinsic excitability in human stem cell–derived neurons.
Journal ArticleDOI
Bright and Fast Multicoloured Voltage Reporters via Electrochromic FRET
Peng Zou,Yongxin Zhao,Adam D. Douglass,Daniel Hochbaum,Daan Brinks,Christopher A. Werley,D. Jed Harrison,Robert E. Campbell,Adam E. Cohen,Adam E. Cohen +9 more
TL;DR: This work identifies linkers and fluorescent protein combinations which report neuronal action potentials in cultured rat hippocampal neurons with a single-trial signal-to-noise ratio from 7 to 9 in a 1 kHz imaging bandwidth at modest illumination intensity.
Journal ArticleDOI
Screening fluorescent voltage indicators with spontaneously spiking HEK cells.
Jeehae Park,Christopher A. Werley,Veena Venkatachalam,Joel M. Kralj,Sulayman D. Dib-Hajj,Stephen G. Waxman,Adam E. Cohen +6 more
TL;DR: A line of non-fluorescent HEK cells that stably express NaV 1.3 and KIR 2.1 and generate spontaneous electrical action potentials are introduced to enable rapid, electrode-free screening of speed and sensitivity of voltage sensitive dyes or fluorescent proteins on a standard fluorescence microscope.
Journal ArticleDOI
Ultrawidefield microscope for high-speed fluorescence imaging and targeted optogenetic stimulation.
TL;DR: The Firefly microscope enables all-optical electrophysiology ('Optopatch') in cultured neurons with a throughput and information content unmatched by other neuronal phenotyping systems, which opens possibilities in disease modeling and phenotypic drug screening.