J
Joel M. Kralj
Researcher at University of Colorado Boulder
Publications - 48
Citations - 3131
Joel M. Kralj is an academic researcher from University of Colorado Boulder. The author has contributed to research in topics: Bacteriorhodopsin & Proteorhodopsin. The author has an hindex of 20, co-authored 47 publications receiving 2705 citations. Previous affiliations of Joel M. Kralj include Boston University & University of Texas at Austin.
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Journal ArticleDOI
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Daniel Hochbaum,Yongxin Zhao,Samouil L. Farhi,Nathan C. Klapoetke,Christopher A. Werley,Vikrant Kapoor,Peng Zou,Joel M. Kralj,Dougal Maclaurin,Niklas Smedemark-Margulies,Jessica L. Saulnier,Gabriella L. Boulting,Christoph Straub,Yong Ku Cho,Michael Melkonian,Gane Ka-Shu Wong,D. Jed Harrison,Venkatesh N. Murthy,Bernardo L. Sabatini,Edward S. Boyden,Robert E. Campbell,Adam E. Cohen +21 more
TL;DR: In this article, a coexpression vector, Optopatch, enabled cross-talk-free genetically targeted all-optical electrophysiology without the use of conventional electrodes.
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Daniel Hochbaum,Yongxin Zhao,Samouil L. Farhi,Christopher A. Werley,Vikrant Kapoor,Peng Zou,Joel M. Kralj,Dougal Maclaurin,Niklas Smedemark-Margulies,Jessica L. Saulnier,Gabriella L. Boulting,Christoph Straub,Michael Melkonian,Gane Ka-Shu Wong,D. Jed Harrison,Venkatesh N. Murthy,Bernardo L. Sabatini,Robert E. Campbell,Adam E. Cohen,Nathan C. Klapoetke,Yong Ku Cho,Edward S. Boyden +21 more
TL;DR: The Optopatch platform enables high-throughput, spatially resolved electrophysiology without the use of conventional electrodes and revealed homeostatic tuning of intrinsic excitability in human stem cell–derived neurons.
Journal ArticleDOI
Optical recording of action potentials in mammalian neurons using a microbial rhodopsin
TL;DR: The endogenous fluorescence of a microbial rhodopsin protein, Archaerhodops in 3 (Arch) from Halorubrum sodomense, expressed in cultured rat hippocampal neurons, exhibited an approximately tenfold improvement in sensitivity and speed over existing protein-based voltage indicators.
Journal ArticleDOI
Electrical Spiking in Escherichia coli Probed with a Fluorescent Voltage-Indicating Protein
TL;DR: Electrical spiking in Escherichia coli was sensitive to chemical and physical perturbations and coincided with rapid efflux of a small-molecule fluorophore, suggesting that bacterial efflux machinery may be electrically regulated.
Journal ArticleDOI
Cardiotoxicity screening with simultaneous optogenetic pacing, voltage imaging and calcium imaging.
Graham Dempsey,Khuram W. Chaudhary,Nicholas Atwater,Cuong Nguyen,Barry S. Brown,John D. McNeish,Adam E. Cohen,Joel M. Kralj +7 more
TL;DR: A cardiotoxicity assay incorporating simultaneous measurement of the action potential (AP) waveform and Ca(2+) transient (CT) in human iPSC-derived cardiomyocytes (CMs) is developed and reported spontaneous and paced APs and CTs with high signal-to-noise ratio and low phototoxicity.