G
Gabriella L. Boulting
Researcher at Harvard University
Publications - 17
Citations - 4639
Gabriella L. Boulting is an academic researcher from Harvard University. The author has contributed to research in topics: Induced pluripotent stem cell & Cellular differentiation. The author has an hindex of 13, co-authored 17 publications receiving 4159 citations. Previous affiliations of Gabriella L. Boulting include Howard Hughes Medical Institute & University of California, Los Angeles.
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Journal ArticleDOI
Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines
Christoph Bock,Evangelos Kiskinis,Evangelos Kiskinis,Griet Verstappen,Griet Verstappen,Hongcang Gu,Gabriella L. Boulting,Zachary D. Smith,Zachary D. Smith,Michael J. Ziller,Michael J. Ziller,Gist F. Croft,Mackenzie W. Amoroso,Derek H. Oakley,Andreas Gnirke,Kevin Eggan,Kevin Eggan,Alexander Meissner,Alexander Meissner +18 more
TL;DR: This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines, and yields a scorecard for quick and comprehensive characterization of pluripotent cell lines.
Journal ArticleDOI
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Daniel Hochbaum,Yongxin Zhao,Samouil L. Farhi,Nathan C. Klapoetke,Christopher A. Werley,Vikrant Kapoor,Peng Zou,Joel M. Kralj,Dougal Maclaurin,Niklas Smedemark-Margulies,Jessica L. Saulnier,Gabriella L. Boulting,Christoph Straub,Yong Ku Cho,Michael Melkonian,Gane Ka-Shu Wong,D. Jed Harrison,Venkatesh N. Murthy,Bernardo L. Sabatini,Edward S. Boyden,Robert E. Campbell,Adam E. Cohen +21 more
TL;DR: In this article, a coexpression vector, Optopatch, enabled cross-talk-free genetically targeted all-optical electrophysiology without the use of conventional electrodes.
Journal ArticleDOI
Intrinsic Membrane Hyperexcitability of Amyotrophic Lateral Sclerosis Patient-Derived Motor Neurons
Brian J. Wainger,Evangelos Kiskinis,Cassidy Mellin,Ole Wiskow,Steve S.W. Han,Jackson Sandoe,Numa P. Perez,Luis A. Williams,Seungkyu Lee,Gabriella L. Boulting,James D. Berry,Robert H. Brown,Merit Cudkowicz,Bruce P. Bean,Kevin Eggan,Kevin Eggan,Clifford J. Woolf,Clifford J. Woolf +17 more
TL;DR: It is shown that hyperexcitability detected by clinical neurophysiological studies of ALS patients is recapitulated in induced pluripotent stem cell-derived motor neurons from patients harboring superoxide dismutase 1 (SOD1), C9orf72, and fused-in-sarcoma mutations.
Journal ArticleDOI
A functionally characterized test set of human induced pluripotent stem cells
Gabriella L. Boulting,Evangelos Kiskinis,Evangelos Kiskinis,Gist F. Croft,Mackenzie W. Amoroso,Derek H. Oakley,Brian J. Wainger,Brian J. Wainger,Damian J. Williams,David J. Kahler,Mariko Yamaki,Mariko Yamaki,Lance S. Davidow,Christopher T Rodolfa,John T. Dimos,Shravani Mikkilineni,Amy B. MacDermott,Clifford J. Woolf,Clifford J. Woolf,Christopher E. Henderson,Hynek Wichterle,Kevin Eggan,Kevin Eggan +22 more
TL;DR: All 16 iPSC lines passed a stringent test of differentiation capacity despite variations in karyotype and in the expression of early pluripotency markers and transgenes.
Journal ArticleDOI
Human Embryonic Stem Cell-Derived Motor Neurons Are Sensitive to the Toxic Effect of Glial Cells Carrying an ALS-Causing Mutation
TL;DR: It is shown that human spinal motor neurons, but not interneurons, are selectively sensitive to the toxic effect of glial cells carrying an ALS-causing mutation in the SOD1 gene, demonstrating the relevance of these non-cell-autonomous effects to human motor neurons.