Christopher Vollmers

TL;DR: It is found that tracts of homozygosity are rarely shared among geographically isolated North American populations, suggesting that assisted gene flow would restore local genetic diversity, and that sustaining diversity in small and isolated populations will require either repeated translocations or restoration of landscape connectivity.

TL;DR: A medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells.

TL;DR: The results support the idea that inversions in D. melanogaster experience natural selection to maintain associations between functionally related alleles to produce complex phenotypic outcomes.

TL;DR: R2C2 is used to sequence and demultiplex 12 million full-length cDNA molecules generated by the 10X Chromium platform from ∼3000 peripheral blood mononuclear cells (PBMCs) and this work generated isoform-level transcriptomes for all single cells and designed a computational workflow to extract paired adaptive immune receptors.

TL;DR: A simplified workflow for amplification of IgG antibody variable regions from hybridoma RNA by a specialized RT-PCR followed by Sanger sequencing is described, which successfully sequenced the variable regions of five mouse monoclonal IgG antibodies and enabled the design of chimeric mouse/human antibody expression plasmids for recombinant antibody production in mammalian cell culture expression systems.