Christopher Vollmers

TL;DR: High-resolution proteomics analysis of immunoglobulin coupled with high-throughput sequencing of transcripts encoding B cell receptors to quantitatively determine the antibody repertoire at the individual clonotype level in the sera of young adults before and after vaccination with trivalent seasonal influenza vaccine reveals unanticipated insights regarding the serological response to influenza vaccination.

TL;DR: Coupling of cycling histone modifications with nearby oscillating transcripts thus established a temporal relationship between enhancers, genes, and transcripts on a genome-wide scale in a mammalian liver, offering a framework for understanding the dynamics of metabolism, circadian clock, and chromatin modifications involved in metabolic homeostasis.

TL;DR: A consensus read sequencing approach that incorporates unique barcode labels on each starting RNA molecule enables accurate quantification of RNA and isotype levels and validated this approach and analyzed the differential response of the antibody repertoire to live-attenuated or trivalent-inactivated influenza vaccination.

TL;DR: Depletion of JARID1a in mammalian cells reduced Per promoter histone acetylation, dampened expression of canonical circadian genes, and shortened the period of circadian rhythms, indicating a nonredundant role in circadian oscillator function.

TL;DR: Application of ATAC-seq to sorted nuclei identifies accessible regions genome-wide and compares favorably with published DNaseI sequencing (DNase-seq) results and it requires less than 50 000 nuclei for accurate identification of accessible genomic regions.