C
Chun-Qing Song
Researcher at University of Massachusetts Medical School
Publications - 31
Citations - 2779
Chun-Qing Song is an academic researcher from University of Massachusetts Medical School. The author has contributed to research in topics: Genome editing & Cas9. The author has an hindex of 19, co-authored 29 publications receiving 2106 citations. Previous affiliations of Chun-Qing Song include Chinese Academy of Sciences & Beijing Normal University.
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Journal ArticleDOI
Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo
Hao Yin,Chun-Qing Song,Joseph R. Dorkin,Lihua Julie Zhu,Yingxiang Li,Qiongqiong Wu,Angela I. Park,Junghoon Yang,Sneha Suresh,Aizhan Bizhanova,Ankit Gupta,Mehmet Fatih Bolukbasi,Stephen Walsh,Roman L. Bogorad,Guangping Gao,Zhiping Weng,Yizhou Dong,Victor Koteliansky,Victor Koteliansky,Scot A. Wolfe,Robert Langer,Wen Xue,Daniel G. Anderson +22 more
TL;DR: The delivery strategy is applied to a mouse model of human hereditary tyrosinemia and it is shown that the treatment generated fumarylacetoacetate hydrolase (Fah)-positive hepatocytes by correcting the causative Fah-splicing mutation and rescued disease symptoms such as weight loss and liver damage.
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Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing
Hao Yin,Chun-Qing Song,Sneha Suresh,Qiongqiong Wu,Stephen Walsh,Luke H. Rhym,Esther Mintzer,Mehmet Fatih Bolukbasi,Lihua Julie Zhu,Kevin J. Kauffman,Haiwei Mou,Alicia Oberholzer,Junmei Ding,Suet-Yan Kwan,Roman L. Bogorad,Timofei S. Zatsepin,Timofei S. Zatsepin,Victor Koteliansky,Scot A. Wolfe,Wen Xue,Robert Langer,Daniel G. Anderson +21 more
TL;DR: This work identifies regions of sgRNA that can be modified while maintaining or enhancing genome-editing activity, and develops an optimal set of chemical modifications for in vivo applications, and shows that a single intravenous injection into mice induces >80% editing of Pcsk9 in the liver.
Journal ArticleDOI
Partial DNA-guided Cas9 enables genome editing with reduced off-target activity.
Hao Yin,Chun-Qing Song,Sneha Suresh,Suet-Yan Kwan,Qiongqiong Wu,Stephen Walsh,Junmei Ding,Roman L. Bogorad,Lihua Julie Zhu,Scot A. Wolfe,Victor Koteliansky,Wen Xue,Robert Langer,Daniel G. Anderson +13 more
TL;DR: It is demonstrated that partial replacement of RNA nucleotide with DNA nucleotides in CRISPR RNA (crRNA) enables efficient gene editing in human cells and significantly reduces off- target genome editing through focused analysis of off-target cleavage, measurement of mismatch tolerance and genome-wide profiling of off -target sites.
Journal ArticleDOI
A compact, high-accuracy Cas9 with a dinucleotide PAM for in vivo genome editing
Alireza Edraki,Aamir Mir,Raed Ibraheim,Ildar Gainetdinov,Yeonsoo Yoon,Chun-Qing Song,Yueying Cao,Judith Gallant,Wen Xue,Jaime A. Rivera-Pérez,Erik J. Sontheimer +10 more
TL;DR: Nme2Cas9 combines all-in-one AAV compatibility, exceptional editing accuracy within cells, and high target site density for in-vivo genome editing applications and expands the single-AAV platform to pre-implanted zygotes for streamlined generation of genome-edited mice.
Journal ArticleDOI
CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
Haiwei Mou,Jordan L. Smith,Lingtao Peng,Hao Yin,Jill Moore,Xiao-Ou Zhang,Chun-Qing Song,Ankur Sheel,Qiongqiong Wu,Deniz M. Ozata,Yingxiang Li,Yingxiang Li,Daniel G. Anderson,Charles P. Emerson,Erik J. Sontheimer,Melissa J. Moore,Zhiping Weng,Zhiping Weng,Wen Xue +18 more
TL;DR: It is shown that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons, adding to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.