C
Clément Nizak
Researcher at PSL Research University
Publications - 32
Citations - 1608
Clément Nizak is an academic researcher from PSL Research University. The author has contributed to research in topics: Phage display & Golgi apparatus. The author has an hindex of 18, co-authored 32 publications receiving 1359 citations. Previous affiliations of Clément Nizak include Rockefeller University & École Normale Supérieure.
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Journal ArticleDOI
Two DNA nanomachines map pH changes along intersecting endocytic pathways inside the same cell
TL;DR: It is shown that two distinct DNA nanomachines can be used simultaneously to map pH gradients along two different but intersecting cellular entry pathways inside the same cell.
Journal ArticleDOI
Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring.
Klaus Eyer,Raphael Clement Li-Ming Doineau,Carlos Castrillon,Carlos Castrillon,Luis Briseño-Roa,Vera Menrath,Guillaume Mottet,Guillaume Mottet,Patrick England,Alexei Godina,Elodie Michele Christine Brient-Litzler,Clément Nizak,Allan Jensen,Andrew D. Griffiths,Jérôme Bibette,Pierre Bruhns,Pierre Bruhns,Jean Baudry +17 more
TL;DR: A simple microfluidic system, DropMap, in which single cells are compartmentalized in tens of thousands of 40-pL droplets and analyzed in two-dimensional droplet arrays using a fluorescence relocation-based immunoassay will not only enable immune monitoring and optimization of immunization and vaccination protocols but also potentiate antibody screening.
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Rab6A and Rab6A' GTPases play non-overlapping roles in membrane trafficking.
Elaine Del Nery,Stéphanie Miserey-Lenkei,Thomas Falguières,Clément Nizak,Ludger Johannes,Franck Perez,Bruno Goud +6 more
TL;DR: It is reported that reduction in the expression of Rab6 isoform using specific small interfering RNA reveals noticeable differences in the Rab 6A and Rab6A′ biological functions, and suggests a novel role for Rab6 a′ as the major Rab 6 isoform regulating previously described Rab6‐dependent transport pathways.
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From Grazing Resistance to Pathogenesis: The Coincidental Evolution of Virulence Factors
Sandrine Adiba,Sandrine Adiba,Clément Nizak,Minus van Baalen,Minus van Baalen,Erick Denamur,Frantz Depaulis +6 more
TL;DR: Experimental proof of the key role of the irp gene in the grazing resistance was evidenced with a mutant strain lacking this gene, suggesting that determinant of virulence may well be originally selected and further maintained for their role in natural habitat: resistance to digestion by free-living protozoa, rather than for virulence per se.
Journal ArticleDOI
High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics
Annabelle Gérard,Adam Woolfe,Guillaume Mottet,Marcel Reichen,Carlos Castrillon,Vera Menrath,Sami Ellouze,Adeline Poitou,Raphael Clement Li-Ming Doineau,Luis Briseño-Roa,Pablo Canales-Herrerias,Pascaline Mary,Gregory Rose,Charina Ortega,Matthieu Delince,Sosthene Essono,Bin Jia,Bruno Iannascoli,Odile Richard-Le Goff,Roshan Kumar,Samantha N. Stewart,Yannick Pousse,Bingqing Shen,Kevin Grosselin,Baptiste Saudemont,Antoine Sautel-Caillé,Alexei Godina,Scott McNamara,Klaus Eyer,Gaël A. Millot,Jean Baudry,Patrick England,Clément Nizak,Allan Jensen,Andrew D. Griffiths,Pierre Bruhns,Colin Brenan +36 more
TL;DR: This work presents a method for high-throughput, single-cell screening of IgG-secreting primary cells to characterize antibody binding to soluble and membrane-bound antigens, and generated 77 recombinant antibodies from the identified sequences.