Clément Nizak

TL;DR: It is shown that two distinct DNA nanomachines can be used simultaneously to map pH gradients along two different but intersecting cellular entry pathways inside the same cell.

TL;DR: A simple microfluidic system, DropMap, in which single cells are compartmentalized in tens of thousands of 40-pL droplets and analyzed in two-dimensional droplet arrays using a fluorescence relocation-based immunoassay will not only enable immune monitoring and optimization of immunization and vaccination protocols but also potentiate antibody screening.

TL;DR: It is reported that reduction in the expression of Rab6 isoform using specific small interfering RNA reveals noticeable differences in the Rab 6A and Rab6A′ biological functions, and suggests a novel role for Rab6 a′ as the major Rab 6 isoform regulating previously described Rab6‐dependent transport pathways.

TL;DR: Experimental proof of the key role of the irp gene in the grazing resistance was evidenced with a mutant strain lacking this gene, suggesting that determinant of virulence may well be originally selected and further maintained for their role in natural habitat: resistance to digestion by free-living protozoa, rather than for virulence per se.

TL;DR: This work presents a method for high-throughput, single-cell screening of IgG-secreting primary cells to characterize antibody binding to soluble and membrane-bound antigens, and generated 77 recombinant antibodies from the identified sequences.