C
Cornel Fraefel
Researcher at University of Zurich
Publications - 157
Citations - 4659
Cornel Fraefel is an academic researcher from University of Zurich. The author has contributed to research in topics: Herpes simplex virus & Amplicon. The author has an hindex of 37, co-authored 150 publications receiving 4309 citations. Previous affiliations of Cornel Fraefel include Max Planck Society & Children's Medical Center of Dallas.
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Helper virus-free transfer of herpes simplex virus type 1 plasmid vectors into neural cells
Cornel Fraefel,Song Song,Filip Lim,Phung Lang,Linda Yu,Yaming Wang,Peter J. Wild,Andalfred I. Geller +7 more
TL;DR: In the absence of helper virus, the vectors efficiently infected rat neural cells in culture or in the brain with minimal cytopathic effects and vector DNA persisted for this period, facilitating studies on neuronal physiology and potential therapeutic applications.
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Herpes simplex virus type 1 DNA amplified as bacterial artificial chromosome in Escherichia coli : rescue of replication-competent virus progeny and packaging of amplicon vectors
Yoshinaga Saeki,Tomotsuga Ichikawa,A Saeki,E A Chiocca,Kurt Tobler,Kurt Tobler,Mathias Ackermann,Xandra O. Breakefield,Cornel Fraefel,Cornel Fraefel +9 more
TL;DR: The capacity of generating infectious and replication-competent HSV-1 progeny following transfection into mammalian cells was restored after insertion of a pac signal into fHSV delta pac, and the resulting amplicon stocks are virtually free of contaminating helper virus.
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Downregulated MicroRNA-200a in Meningiomas Promotes Tumor Growth by Reducing E-Cadherin and Activating the Wnt/β-Catenin Signaling Pathway
Okay Saydam,Yiping Shen,Thomas Wurdinger,Thomas Wurdinger,Ozlem Senol,Elvan Boke,Marianne James,Bakhos A. Tannous,Anat Stemmer-Rachamimov,Ming Yi,Robert M. Stephens,Cornel Fraefel,James F. Gusella,Anna M. Krichevsky,Xandra O. Breakefield +14 more
TL;DR: In this paper, the effects of one downregulated miRNA, miR-200a, on tumor growth were investigated. And they showed that miR200a appears to act as a multifunctional tumor suppressor miRNA in meningiomas through effects on the E-cadherin and Wnt/β-catenin signaling pathways.
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Improved Helper Virus-Free Packaging System for HSV Amplicon Vectors Using an ICP27-Deleted, Oversized HSV-1 DNA in a Bacterial Artificial Chromosome
Yoshinaga Saeki,Yoshinaga Saeki,Cornel Fraefel,Tomotsugu Ichikawa,Xandra O. Breakefield,E. Antonio Chiocca +5 more
TL;DR: Genetic modifications of HSV-1 genomes can be routinely achieved in Escherichia coli, either by homologous or site-specific recombination, and elimination of replication-competent helper virus from HSv-1 amplicon vector stocks further improves safety in gene transfer applications.
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Immediate-early RNA 2.9 and early RNA 2.6 of bovine herpesvirus 1 are 3' coterminal and encode a putative zinc finger transactivator protein.
TL;DR: BHV-1 seems to have evolved a subtle mechanism to ensure the continued synthesis of p135 while turning off IER4.2, which encodes p180, the herpes simplex virus type 1 ICP4 homolog.