D
Darren J. Hart
Researcher at University of Grenoble
Publications - 79
Citations - 4555
Darren J. Hart is an academic researcher from University of Grenoble. The author has contributed to research in topics: Polymerase & Protein subunit. The author has an hindex of 27, co-authored 79 publications receiving 4120 citations. Previous affiliations of Darren J. Hart include Unit of Virus Host Cell Interactions & Forschungszentrum Jülich.
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Journal ArticleDOI
The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit
Alexandre Dias,Denis Bouvier,Thibaut Crépin,Andrew A. McCarthy,Darren J. Hart,Florence Baudin,Stephen Cusack,Rob W.H. Ruigrok +7 more
TL;DR: In this paper, the amino-terminal 209 residues of the PA subunit contain the active site of the endonuclease active site, which is shown to be strongly activated by manganese ions, matching observations reported for the intact trimeric polymerase.
Journal ArticleDOI
The Structural Basis for CAP Binding by Influenza Virus Polymerase Subunit Pb2.
Delphine Guilligay,Franck Tarendeau,Patricia Resa-Infante,Rocío Coloma,Thibaut Crépin,Peter Sehr,Joe Lewis,Rob W.H. Ruigrok,Juan Ortín,Darren J. Hart,Stephen Cusack +10 more
TL;DR: Binding and functional studies with point mutants confirm that the identified site is essential for cap binding in vitro and cap-dependent transcription in vivo by the trimeric polymerase complex, and will allow efficient structure-based design of new anti-influenza compounds inhibiting viral transcription.
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Cooperative binding of two acetylation marks on a histone tail by a single bromodomain
Jeanne Morinière,Sophie Rousseaux,Ulrich Steuerwald,Montserrat Soler-López,Sandrine Curtet,Anne-Laure Vitte,Jérôme Govin,Jonathan Gaucher,Karin Sadoul,Darren J. Hart,Jeroen Krijgsveld,Saadi Khochbin,Christoph W. Müller,Carlo Petosa +13 more
TL;DR: A new mechanism for the combinatorial readout of histone modifications in which a single effector module engages two marks on a histone tail as a composite binding epitope is described.
Journal ArticleDOI
Structural insight into cap-snatching and RNA synthesis by influenza polymerase
Stefan Reich,Delphine Guilligay,Alexander Pflug,Hélène Malet,Imre Berger,Thibaut Crépin,Darren J. Hart,Thomas Lunardi,Max H. Nanao,Rob W.H. Ruigrok,Stephen Cusack +10 more
TL;DR: Crystal structures of bat influenza A and human influenza B polymerases (FluA and FluB), bound to the viral RNA promoter, are used to give mechanistic insight into these distinct processes.
Journal ArticleDOI
Structure and Nuclear Import Function of the C- Terminal Domain of Influenza Virus Polymerase Pb2 Subunit
Franck Tarendeau,Julien Boudet,Delphine Guilligay,Philippe J. Mas,Catherine M. Bougault,Sébastien Boulo,Florence Baudin,Rob W.H. Ruigrok,Nathalie Daigle,Jan Ellenberg,Stephen Cusack,Jean-Pierre Simorre,Darren J. Hart +12 more
TL;DR: It is shown that both the domain and the full-length PB2 subunit are efficiently imported into the nucleus dependent on a previously overlooked bipartite nuclear localization sequence (NLS).