D
David Bikard
Researcher at Pasteur Institute
Publications - 84
Citations - 8898
David Bikard is an academic researcher from Pasteur Institute. The author has contributed to research in topics: CRISPR & Gene. The author has an hindex of 29, co-authored 69 publications receiving 6974 citations. Previous affiliations of David Bikard include Massachusetts Institute of Technology & McGovern Institute for Brain Research.
Papers
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Journal ArticleDOI
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Wenyan Jiang,David Bikard,David Daniel Cox,David Daniel Cox,Feng Zhang,Feng Zhang,Luciano A. Marraffini +6 more
TL;DR: The exhaustively analyze dual-RNA:Cas9 target requirements to define the range of targetable sequences and show strategies for editing sites that do not meet these requirements, suggesting the versatility of this technique for bacterial genome engineering.
Journal ArticleDOI
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
TL;DR: A Cas9 nuclease mutant that retains DNA-binding activity and can be engineered as a programmable transcription repressor by preventing the binding of the RNA polymerase to promoter sequences or as a transcription terminator by blocking the running RNAP is described.
Journal ArticleDOI
Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials
David Bikard,Chad W. Euler,Wenyan Jiang,Philip M Nussenzweig,Gregory W. Goldberg,Xavier Duportet,Vincent A. Fischetti,Luciano A. Marraffini +7 more
TL;DR: It is shown that Cas9, reprogrammed to target virulence genes, kills virulent, but not avirulent, Staphylococcus aureus, and that CRISPR-Cas9 antimicrobials function in vivo to kill S. aUREus in a mouse skin colonization model.
Patent
CRISPR-Cas component systems, methods and compositions for sequence manipulation
Feng Zhang,David Bikard,Le Cong,David Benjamin Turitz Cox,Patrick D. Hsu,Wenyan Jiang,Shauiliang Lin,Luciano A. Marraffini,Randall Jeffrey Platt,Fei Ran,Neville E. Sanjana +10 more
TL;DR: In this paper, the authors provide a system, methods, and compositions for manipulation of sequences and/or activities of target sequences, including vectors and vector systems, some of which encode one or more components of a CRISPR complex, and methods for the design and use of such vectors.
Journal ArticleDOI
PhageTerm: a tool for fast and accurate determination of phage termini and packaging mechanism using next-generation sequencing data.
Julian R. Garneau,Julian R. Garneau,Florence Depardieu,Louis-Charles Fortier,David Bikard,Marc Monot,Marc Monot,Marc Monot +7 more
TL;DR: A theoretical and statistical framework to determine DNA termini and phage packaging mechanisms using NGS data is developed and validated using a set of phages with well-established packaging mechanisms representative of the termini diversity.